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Overexpression of yeast S-adenosylmethionine synthetase metK in Streptomyces actuosus leads to increased production of nosiheptide

  • Applied Genetics and Molecular Biotechnology
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Abstract

S-Adenosylmethionine (SAM) is synthesized via the metabolic reaction involving adenosine triphosphate and l-methionine that is catalyzed by the enzyme S-adenosyl-l-methionine synthetase (SAM-s) and encoded by the gene metK. In the present study, metK with the absence of introns from Saccharomyces cerevisiae was introduced into Streptomyces actuosus, a nosiheptide (Nsh) producer. Intracellular SAM levels were determined by high-pressure liquid chromatography. Through optimizing the nutrient content of the medium, it was shown that increased SAM production induced by the overexpression of SAM-s leads to an increase in the intracellular cysteine pool and overproduction of Nsh in S. actuosus. This investigation shows that increased SAM promotes the elevated production of the non-ribosomal thiopeptide Nsh in Streptomyces sp.

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Authors and Affiliations

  1. School of Pharmacy, Fudan University, Yi Xue Yuan Road 138, Shanghai, 200032, China

    Xincheng Zhang, Meiqing Fen, Xunlong Shi & Pei Zhou

  2. Laboratory of Microbial Metabolism, School of Life Science and Biotechnology, Shanghai Jiaotong University, Shanghai, 200030, China

    Linquan Bai

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  1. Xincheng Zhang
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  2. Meiqing Fen
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  3. Xunlong Shi
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  4. Linquan Bai
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  5. Pei Zhou
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Correspondence to Linquan Bai or Pei Zhou.

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Zhang, X., Fen, M., Shi, X. et al. Overexpression of yeast S-adenosylmethionine synthetase metK in Streptomyces actuosus leads to increased production of nosiheptide. Appl Microbiol Biotechnol 78, 991–995 (2008). https://doi.org/10.1007/s00253-008-1394-5

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  • DOI: https://doi.org/10.1007/s00253-008-1394-5

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