Abstract
The anode potential in microbial fuel cells controls both the theoretical energy gain for the microorganisms as the output of electrical energy. We operated three reactors fed with acetate continuously at a poised anode potential of 0 (R 0), −200 (R −200) and −400 (R −400) mV versus Ag/AgCl and investigated the resulting bacterial activity. The anode potential had no influence on the start-up time of the three reactors. During a 31-day period, R −200 produced 15% more charge compared to R 0 and R −400. In addition, R −200 had the highest maximal power density (up to 199 W m−3 total anode compartment during polarization) but the three reactors evolved to the same power density at the end of the experimental period. During polarization, only the current of R −400 levelled off at an anode potential of −300 mV versus Ag/AgCl. The maximum respiration rate of the bacteria during batch tests was also considerably lower for R −400. The specific biomass activity however, was the highest for R −400 (6.93 g chemical oxygen demand g−1 biomass-volatile suspended solids (VSS) d−1 on day 14). This lowered during the course of the experiment due to an increase of the biomass concentration to an average level of 578 ± 106 mg biomass-VSS L−1 graphite granules for the three reactors. This research indicated that an optimal anode potential of −200 mV versus Ag/AgCl exists, regulating the activity and growth of bacteria to sustain an enhanced current and power generation.
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Aelterman P, Rabaey K, Pham HT, Boon N, Verstraete W (2006) Continuous electricity generation at high voltages and currents using stacked microbial fuel cells. Environ Sci Technol 40:3388–3394
Allen RM, Bennetto HP (1993) Microbial fuel-cells—electricity production from carbohydrates. Appl Biochem Biotechnol 39:27–40
Bond DR, Lovley DR (2003) Electricity production by Geobacter sulfurreducens attached to electrodes. Appl Environ Microbiol 69:1548–1555
Chaudhuri SK, Lovley DR (2003) Electricity generation by direct oxidation of glucose in mediatorless microbial fuel cells. Nat Biotechnol 21:1229–1232
Findlay RH, King GM, Watling L (1989) Efficacy of phospholipid analysis in determining microbial biomass in sediments. Appl Environ Microbiol 55:2888–2893
Finkelstein DA, Tender LM, Zeikus JG (2006) Effect of electrode potential on electrode-reducing microbiota. Environ Sci Technol 40:6990–6995
Freguia S, Rabaey K, Yuan ZG, Keller J (2007) Electron and carbon balances in microbial fuel cells reveal temporary bacterial storage behavior during electricity generation. Environ Sci Technol 41:2915–2921
Gapes D, Pratt S, Yuan ZG, Keller J (2003) Online titrimetric and off-gas analysis for examining nitrification processes in wastewater treatment. Water Res 37:2678–2690
Gorby YA, Yanina S, McLean JS, Rosso KM, Moyles D, Dohnalkova A, Beveridge TJ, Chang IS, Kim BH, Kim KS, Culley DE, Reed SB, Romine MF, Saffarini DA, Hill EA, Shi L, Elias DA, Kennedy DW, Pinchuk G, Watanabe K, Ishii S, Logan B, Nealson KH, Fredrickson JK (2006) Electrically conductive bacterial nanowires produced by Shewanella oneidensis strain MR-1 and other microorganisms. Proc Natl Acad Sci U S A 103:11358–11363
Hernandez ME, Newman DK (2001) Extracellular electron transfer. Cell Mol Life Sci 58:1562–1571
Kim HJ, Park HS, Hyun MS, Chang IS, Kim M, Kim BH (2002) A mediator-less microbial fuel cell using a metal reducing bacterium, Shewanella putrefaciens. Enzyme Microb Technol 30:145–152
Kim JR, Min B, Logan BE (2005) Evaluation of procedures to acclimate a microbial fuel cell for electricity production. Appl Microbiol Biotechnol 68:23–30
Liu H, Cheng SA, Logan BE (2005) Production of electricity from acetate or butyrate using a single-chamber microbial fuel cell. Environ Sci Technol 39:658–662
Logan BE, Hamelers B, Rozendal R, Schrorder U, Keller J, Freguia S, Aelterman P, Verstraete W, Rabaey K (2006) Microbial fuel cells: methodology and technology. Environ Sci Technol 40:5181–5192
Park DH, Zeikus JG (2003) Improved fuel cell and electrode designs for producing electricity from microbial degradation. Biotechnol Bioeng 81:348–355
Pham HT, Boon N, Aelterman P, Clauwaert P, De Schamphelaire L, Vanhaecke L, De Maeyer K, Höfte M, Verstraete W, Rabaey K (2008) Metabolites produced by Pseudomonas sp. enable a Gram-positive bacterium to achieve extracellular electron transfer. Appl Microbiol Biotechnol 77(5):1119–29 DOI https://doi.org/10.1007/s00253-007-1248-6
Picioreanu C, Head IM, Katuri KP, van Loosdrecht MCM, Scott K (2007) A computational model for biofilm-based microbial fuel cells. Water Res 41:2921–2940
Porges N, Jasewicz L, Hoover SR (1953) Aerobic treatment of dairy wastes. Appl Microbiol 1:262–270
Pratt S, Yuan ZG, Gapes D, Dorigo M, Zeng RJ, Keller J (2003) Development of a novel titration and off-gas analysis (TOGA) sensor for study of biological processes in wastewater treatment systems. Biotechnol Bioeng 81:482–495
Rabaey K, Lissens G, Siciliano SD, Verstraete W (2003) A microbial fuel cell capable of converting glucose to electricity at high rate and efficiency. Biotechnol Lett 25:1531–1535
Rabaey K, Boon N, Siciliano SD, Verhaege M, Verstraete W (2004) Biofuel cells select for microbial consortia that self-mediate electron transfer. Appl Environ Microbiol 70:5373–5382
Rabaey K, Boon N, Hofte M, Verstraete W (2005a) Microbial phenazine production enhances electron transfer in biofuel cells. Environ Sci Technol 39:3401–3408
Rabaey K, Clauwaert P, Aelterman P, Verstraete W (2005b) Tubular microbial fuel cells for efficient electricity generation. Environ Sci Technol 39:8077–8082
Rabaey K, Ossieur W, Verhaege M, Verstraete W (2005c) Continuous microbial fuel cells convert carbohydrates to electricity. Water Sci Technol 52:515–523
Rabaey K, Van de Sompel K, Maignien L, Boon N, Aelterman P, Clauwaert P, De Schamphelaire L, Pham HT, Vermeulen J, Verhaege M, Lens P, Verstraete W (2006) Microbial fuel cells for sulfide removal. Environ Sci Technol 40:5218–5224
Reguera G, McCarthy KD, Mehta T, Nicoll JS, Tuominen MT, Lovley DR (2005) Extracellular electron transfer via microbial nanowires. Nature 435:1098–1101
Schroder U (2007) Anodic electron transfer mechanisms in microbial fuel cells and their energy efficiency. Phys Chem Chem Phys 9:2619–2629
Straub KL, Schink B (2004) Ferrihydrite-dependent growth of Sulfurospirillum deleyianum through electron transfer via sulfur cycling. Appl Environ Microbiol 70:5744–5749
Thauer RK, Jungermann K, Decker K (1977) Energy-conservation in chemotropic anaerobic bacteria. Bacteriol Rev 41:100–180
Zinatizadeh AAL, Mohamed AR, Najafpour GD, Hasnain Isa M, Nasrollahzadeh H (2006) Kinetic evaluation of palm oil mill effluent digestion in a high rate up-flow anaerobic sludge fixed film bioreactor. Process Biochem 41:1038–1046
Acknowledgements
This research was funded by the Research Foundation—Flanders by the FWO (project G.0172.05 and credits for a stay abroad (V4/20B-4672)). Korneel Rabaey is supported by the UQ Postdoctoral Research Fellow Scheme, the Early Career Researcher scheme and the Australian Research Council (DP0666927). The useful comments of Tom Defoirdt, Lieven Wittenbolle, Peter Clauwaert, Liesje DeSchamphelaire, Gorge Ignacio and Nico Boon were highly appreciated.
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Aelterman, P., Freguia, S., Keller, J. et al. The anode potential regulates bacterial activity in microbial fuel cells. Appl Microbiol Biotechnol 78, 409–418 (2008). https://doi.org/10.1007/s00253-007-1327-8
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DOI: https://doi.org/10.1007/s00253-007-1327-8