Abstract
In the global ocean nitrogen cycle, the anaerobic ammonium-oxidizing (anammox) process is recognized as important. In this study, we established an enrichment culture of marine anammox bacteria (MAB) in a column-type reactor. The reactor, which included a porous polyester non-woven fabric that had been placed at the sea floor in advance for enrichment, was continuously fed with NH4Cl and NaNO2 for more than 1 year. Anammox activity in the MAB reactor was confirmed by 15N tracer analysis using 15NH4Cl and Na14NO2. We identified two 16S rRNA genes in the amplified DNA fragments derived from MAB, which were highly homologous with those from Candidatus “Scalindua wagneri” and an uncultured planctomycete clone. Fluorescence in situ hybridization analysis using an anammox-specific probe also confirmed that MAB predominated in the reactor. To our knowledge, this is the first report on the establishment of an enrichment culture of anammox bacteria from the marine environment using a continuous culture system.
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Acknowledgments
This study was financially supported by the grant of Collaboration of Regional Entities for the Advancement of Technological Excellence from Japan Science and Technology Agency. We wish to thank Professor K. Okumura and Dr. K. Sugimura of Mie University for FISH analysis and Mr. T. Doi of Nishihara Environment Technology, and M. W. Benson of Mie University for helpful discussion.
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Nakajima, J., Sakka, M., Kimura, T. et al. Enrichment of anammox bacteria from marine environment for the construction of a bioremediation reactor. Appl Microbiol Biotechnol 77, 1159–1166 (2008). https://doi.org/10.1007/s00253-007-1247-7
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DOI: https://doi.org/10.1007/s00253-007-1247-7