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Aspergillus flavus hydrolases: their roles in pathogenesis and substrate utilization

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Abstract

Aspergillus flavus is a fungus that principally obtains resources for growth in a saprophytic mode. Yet, it also possesses the characteristics of an opportunistic pathogen with a wide, non-specific host range (plants, animals, and insects). It has attained a high level of agricultural significance due to production of the carcinogen aflatoxin, which significantly reduces the value of contaminated crops. To access a large variety of nutrient substrates and penetrate host tissues, A. flavus possesses the capacity to produce numerous extracellular hydrolases. Most work on A. flavus hydrolases has focused on the serine and metalloproteinases, pectinase P2c, and amylase. Many hydrolases are presumed to function in polymer degradation and nutrient capture, but the regulation of hydrolase secretion is complex and substrate dependent. Proteinases are employed not only to help access protein substrates, such as elastin that is found in mammals and insects, but may also play roles in fungal defense and virulence. Secretion of the endopolygalacturonase P2c is strongly correlated with isolate virulence (against plants) and maceration of cotton boll tissues. In some hosts, secretion of α-amylase is critical for starch digestion and may play a critical role in induction of aflatoxin biosynthesis. Despite a significant body of work, much remains to be learned about hydrolase production and utilization by A. flavus. This information may be critical for the formulation of successful strategies to control aflatoxin contamination in affected commodities.

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References

  • Bouzid O, Record E, Asther M, Haon M, Navarro D, Asther M, Lesage-Meessen L (2006) Exploration of members of Aspergillus sections Nigri, Flavi and Terrei for feruloyl esterase production. Can J Microbiol 52:886–892

    Article  CAS  PubMed  Google Scholar 

  • Brown RL, Cleveland TE, Cotty PJ, Mellon JE (1992) Spread of Aspergillus flavus in cotton bolls, decay of intercarpellary membranes, and production of fungal pectinases. Phytopathology 82:462–467

    Article  CAS  Google Scholar 

  • Brown RL, Chen ZY, Cleveland TE, Cotty PJ, Cary JW (2001) Variation in in vitro α-amylase and protease activity is related to the virulence of Aspergillus flavus isolates. J Food Prot 64:401–404

    Article  CAS  PubMed  Google Scholar 

  • Cleveland TE, Cotty PJ (1991) Invasiveness of Aspergillus flavus isolates in wounded cotton bolls is associated with production of a specific fungal polygalacturonase. Phytopathology 81:155–158

    Article  CAS  Google Scholar 

  • Cotty PJ, Cleveland TE, Brown RL, Mellon JE (1990) Variation in polygalacturonase production among Aspergillus flavus isolates. Appl Environ Microbiol 56:3885–3887

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Cotty PJ, Bayman P, Egel DS, Elias KS (1994) Agriculture, aflatoxins and Aspergillus. In: Powell KA (ed) The genus Aspergillus. Plenum, New York, pp 1–27

    Google Scholar 

  • de Oliveira Carvahlo P, Contesini FJ, Bizaco R, Alves Macedo G (2005) Kinetic properties and enantioselectivity of the lipases produced by four Aspergillus species. Food Biotechnol 19:183–192

    Article  CAS  Google Scholar 

  • de Vries RP, Visser J (2001) Aspergillus enzymes involved in degradation of plant cell wall polysaccharides. Microbiol Mol Biol Rev 65:497–522

    Article  PubMed  PubMed Central  Google Scholar 

  • Dowd PF (1992) Insect interaction with mycotoxin-producing fungi and their hosts. In: Bhatnagar D, Lillehoj EB, Arora DK (eds) Mycotoxins in ecological systems, vol 5. Marcel Dekker, New York, pp 137–155

    Google Scholar 

  • Fakhoury AM, Woloshuk CP (1999) Amy1, the α-amylase gene of Aspergillus flavus: involvement in aflatoxin biosynthesis in maize kernels. Phytopathology 89:908–914

    Article  CAS  PubMed  Google Scholar 

  • Fakhoury AM, Woloshuk CP (2001) Inhibition of growth of Aspergillus flavus and fungal α-amylases by a lectin-like protein from Lablab purpureus. Mol Plant Microb Interact 14:955–961

    Article  CAS  Google Scholar 

  • Guo BZ, Russin JS, Cleveland TE, Brown RL, Damann KE (1996) Evidence for cutinase production by Aspergillus flavus and its possible role in infection of corn kernels. Phytopathology 86:824–829

    Article  CAS  Google Scholar 

  • Hedayati MT, Pasqualotto AC, Warn PA, Bowyer P, Denning DW (2007) Aspergillus flavus: human pathogen, allergen and mycotoxin producer. Microbiology 153:1677–1692

    Article  CAS  PubMed  Google Scholar 

  • Impoolsup A, Bhumiratana A, Flegel TW (1981) Isolation of alkaline and neutral proteases from Aspergillus flavus var. columnaris, a soy sauce koji mold. Appl Environ Microbiol 42:619–628

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Kolattukudy PE, Lee JD, Rogers LM, Zimmerman P, Ceselski S, Fox B, Stein B, Copelan EA (1993) Evidence for possible involvement of an elastinolytic serine protease in aspergillosis. Infect Immun 61:2357–2368

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Kothary MH, Chase T Jr, MacMillan JD (1984) Correlation of elastase production by some strains of Aspergillus fumigatus with ability to cause invasive aspergillosis in mice. Infect Immun 43:320–325

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Long K, Ghazali HM, Ariff A, Che Man Y, Bucke C (1998) Substrate preference of mycelium-bound lipase from a strain of Aspergillus flavus Link. Biotechnol Let 20:369–372

    Article  CAS  Google Scholar 

  • Machida M, Asai K, Sano M et al (2005) Genome sequencing and analysis of Aspergillus oryzae. Nature 438:1157–1161

    Article  PubMed  Google Scholar 

  • Mellon JE, Cotty PJ (1995) Expression of elastinolytic activity among isolates in Aspergillus section Flavi. Mycopathologia 131:115–120

    Article  CAS  PubMed  Google Scholar 

  • Mellon JE, Cotty PJ (1996) Purification and partial characterization of an elastinolytic proteinase from Aspergillus flavus culture filtrates. Appl Microbiol Biotechnol 46:138–142

    Article  CAS  Google Scholar 

  • Mellon JE, Cotty PJ (1998) Effects of oilseed storage proteins on aflatoxin production by Aspergillus flavus. J Am Oil Chem Soc 75:1085–1089

    Article  CAS  Google Scholar 

  • Mellon JE, Cotty PJ (1999) Raffinose content may influence cottonseed susceptibility to aflatoxin contamination. J Am Oil Chem Soc 76:883–886

    Article  CAS  Google Scholar 

  • Mellon JE, Cotty PJ (2004) Expression of pectinase activity among Aspergillus flavus isolates from southwestern and southeastern United States. Mycopathologia 157:333–338

    Article  CAS  PubMed  Google Scholar 

  • Mellon JE, Cotty PJ (2007) Preliminary purification and characterization of a xylanase activity from Aspergillus flavus. Proceedings of the beltwide cotton conferences, p 147–152

  • Mellon JE, Cotty PJ, Dowd MK (2000) Influence of lipids with and without other cottonseed reserve materials on aflatoxin B1 production by Aspergillus flavus. J Agric Food Chem 48:3611–3615

    Article  CAS  PubMed  Google Scholar 

  • Mellon JE, Dowd MK, Cotty PJ (2002) Time course study of substrate utilization by Aspergillus flavus in medium simulating corn (Zea mays) kernels. J Agric Food Chem 50:648–652

    Article  CAS  PubMed  Google Scholar 

  • Mellon JE, Dowd MK, Cotty PJ (2005) Substrate utilization by Aspergillus flavus in inoculated whole corn kernels and isolated tissues. J Agric Food Chem 53:2351–2357

    Article  CAS  PubMed  Google Scholar 

  • Olutiola PO, Cole OO (1980) Extracellular invertase from Aspergillus flavus. Physiol Plant 50:26–31

    Article  CAS  Google Scholar 

  • Payne GA, Nierman WC, Wortman JR, Pritchard BL, Brown D, Dean RA, Bhatnagar D, Cleveland TE, Machida M, Yu J (2006) Whole genome comparison of Aspergillus flavus and A. oryzae. Med Mycol 44:S9–S11

    Article  CAS  PubMed  Google Scholar 

  • Ramesh MV, Koluttukudy PE (1996) Disruption of the serine proteinase gene (sep) in Aspergillus flavus leads to a compensatory increase in the expression of a metalloproteinase gene (mep20). J Bacteriol 178:3899–3907

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Ramesh MV, Sirakova T, Kolattukudy PE (1994) Isolation, characterization, and cloning of cDNA and the gene for an elastinolytic serine proteinase from Aspergillus flavus. Infect Immun 62:79–85

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Rhodes JC, Amlung TW, Miller MS (1990) Isolation and characterization of an elastinolytic proteinase from Aspergillus flavus. Infect Immun 58:2529–2534

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Sheih MT, Brown RL, Whitehead MP, Cary JW, Cotty PJ, Cleveland TE, Dean RA (1997) Molecular genetic evidence for the involvement of a specific polygalacturonase, P2c, in the invasion and spread of Aspergillus flavus in cotton bolls. Appl Environ Microbiol 63:3548–3552

    Article  Google Scholar 

  • Stephenson LW, Russell TE (1974) The association of Aspergillus flavus with hemipterous and other insects infesting cotton bracts and foliage. Phytopathology 64:1502–1506

    Article  Google Scholar 

  • St. Leger RJ, Joshi L, Roberts DW (1997) Adaptation of proteases and carbohydrases of saprophytic, phytopathogenic and entomopathogenic fungi to the requirements of their ecological niches. Microbiology 143:1983–1992

    Article  CAS  Google Scholar 

  • St. Leger RJ, Screen SE, Shams-Pirzadeh B (2000) Lack of host specialization in Aspergillus flavus. Appl Environ Microbiol 66:320–324

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Wang ZG, Tong Z, Cheng SY, Cong LM (1993) Study on pectinase and sclerotium producing abilities of two kinds of Aspergillus flavus isolates from Zhejiang. Mycopathologia 121:163–168

    Article  CAS  Google Scholar 

  • Whitehead MP, Sheih MT, Cleveland TE, Cary JW, Dean RA (1995) Isolation and characterization of polygalacturonase genes (pecA and pecB) from Aspergillus flavus. Appl Environ Microbiol 61:3316–3322

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Woloshuk CP, Cavaletto JR, Cleveland TE (1997) Inducers of aflatoxin biosynthesis from colonized maize kernels are generated by an amylase activity from Aspergillus flavus. Phytopathology 87:164–169

    Article  CAS  PubMed  Google Scholar 

  • Wu F (2004) Mycotoxin risk assessment for the purpose of setting international regulatory standards. Environ Sci Technol 38:4049–4055

    Article  CAS  PubMed  Google Scholar 

  • Yoshikawa K, Yamamoto K, Okada S (1993a) Isolation of Aspergillus flavus MO-5 producing two types of intracellular α-D-xylosidases: purification and characterization of α-D-xylosidase I. Biosci Biotechnol Biochem 57:1275–1280

    Article  CAS  PubMed  Google Scholar 

  • Yoshikawa K, Yamamoto K, Okada S (1993b) Purification and characterization of an intracellular α-D-xylosidase II from Aspergillus flavus MO-5. Biosci Biotechnol Biochem 57:1281–1285

    Article  CAS  PubMed  Google Scholar 

  • Yu J, Mohaved SM, Bhatnagar D, Cleveland TE (2003) Substrate-induced lipase gene expression and aflatoxin production in Aspergillus parasiticus and Aspergillus flavus. J Appl Microbiol 95:1334–1342

    Article  CAS  PubMed  Google Scholar 

  • Yu J, Whitelaw CA, Nierman WC, Bhatnagar D, Cleveland TE (2004) Aspergillus flavus expressed sequence tags for identification of genes with putative roles in aflatoxin contamination of crops. FEMS Microbiol Let 237:333–340

    Google Scholar 

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Authors and Affiliations

  1. US Department of Agriculture, Agricultural Research Service, Southern Regional Research Center, P. O. Box 19687, New Orleans, LA, 70179, USA

    Jay E. Mellon & Michael K. Dowd

  2. US Department of Agriculture, Agricultural Research Service, Department of Plant Pathology, University of Arizona, 1140 E. South Campus Dr., Tucson, AZ, 85721, USA

    Peter J. Cotty

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  1. Jay E. Mellon
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  2. Peter J. Cotty
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  3. Michael K. Dowd
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Correspondence to Jay E. Mellon.

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Mellon, J.E., Cotty, P.J. & Dowd, M.K. Aspergillus flavus hydrolases: their roles in pathogenesis and substrate utilization. Appl Microbiol Biotechnol 77, 497–504 (2007). https://doi.org/10.1007/s00253-007-1201-8

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