Abstract
Probiotics and bovine colostrums had been proven to be beneficial for human health. Lactobacillus rhamnosus ZDY114 and anti-Helicobacter pylori bovine colostrums were used for the preparation of microecological additives, and their synergistic antidigestion effect in the simulated gastrointestinal tract (in vitro) was investigated. Either L. rhamnosus or purified IgG from immune colostrums was very sensitive in simulated gastric environment and slightly sensitive in simulated intestinal tract. No viable counts were recovered from the solution of dissolved freeze-dried powder (7.14 log10 CFU/ml) of L. rhamnosus when digested at pH 3.0 with pepsin for 30 min. Activity of purified IgGs from immune colostrums could not be detected when digested at pH 3.0 with pepsin for 30 min; 29% titer could be detected when digested at pH 8.0 with trypsin for 5 h. The IgGs in nonpurified immune colostrums presented stronger resistance against gastrointestinal digestion than purified IgGs. Moreover, the combination of L. rhamnosus ZDY114 and immune colostrums strengthened their antidigestion ability. Even under pH 3.0, 4.0 with pepsin, the titer of anti-HP IgG maintained above 123 and 83.3%, respectively. Similarly, that titer was above 93.3% when digested at pH 8.0 with trypsin for 4.5 h. In conclusion, L. rhamnosus and anti-Helicobacter pylori bovine colostrums had synergistic antidigestion effect in simulated gastrointestinal tract (in vitro).
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Explore related subjects
Discover the latest articles and news from researchers in related subjects, suggested using machine learning.References
Alander M, Korpela R, Saxelin M, Vilpponen-Salmela T, Mattila-Sandholm T, von Wright A (1997) Recovery of L. rhamnosus GG from human colonic biopsies. Lett Appl Microbiol 24:361–364
de Rham O, Isliker H (1977) Proteolysis of bovine immunoglobulins. Int Arch Allergy Appl Immun 55:61–69
Dividson GP (1996) Passive protection against diarrhea disease. J Pediatr Gastroenterol Nutr 23:207–212
Elo S, Saxelin M, Salminen S (1991) Attachment of L. casei strain GG to human colon carcinoma cell line Caco-2: comparison with other dairy strains. Lett Appl Microbiol 13:154–156
Galdeano CM, Perdigón G (2004) Role of viability of probiotic strains in their persistence in the gut and in mucosal immune stimulation. J Appl Microbiol 97(9):673–681
Goldman AS (1989) Immunologic supplementation of cow’s milk formulations. Bull Int Dairy Fed 244:38–42
Guerra NP, Rua ML, Pastrana L (2001) Nutritional factors affecting the production of two bacteriocins from lactic acid bacteria on whey. Int J Food Microbiol 70:267–281
Hammarstr L, Gardulf A, Hammarstr V, Janson A, Lindberg K, Smith CIE (1994) Systemic and topical immunoglobulin treatment in immunocompromised patients. Immunol Rev 139:43–47
International Dairy Federation (1993) Indigenous antimicrobial agents of milk: recent developments. Proceedings of the IDF Seminar held in Uppsala, Sweden, 31 August and 1 September 1993, pp 14–51
Korhonen H, Pihlanto-leppo A, Rantaamaki P, Tupasela T (1998) The functional and biological properties of whey proteins: prospects for the development of functional foods. Agric Food Sci Finl 7:283–296
Korhonen H, Marnila P, Gill HS (2000a) Bovine milk antibodies for health. Br J Nutr 84 (Suppl 1):135–146
Korhonen H, Marnila P, Gill HS (2000b) Milk immunoglobulins and complement factors. Br J Nutr. 84 (Suppl 1):75–80
Kuisma J, Mentula S, Jarvinen H, Kahri A, Saxelin M, Farkkila M (2003) Effect of L. rhamnosus GG on ileal pouch inflammation and microbial flora. Aliment Pharmacol Ther 17:509–515
Leblanc J, Fliss I, Matar C (2004) Induction of a humoral immune response following an Escherichia coli O157:H7 infection with an immunomodulatory peptidic fraction derived from L. helveticus-fermented milk. Clin Diagn Lab Immunol 11:1171–1181
Lee YK, Puong KY (2002) Competition for adhesion between probiotics and human gastrointestinal pathogens in the presence of carbohydrate. Br J Nutr 88 (suppl 1):101–108
Lee YK, Puong KY, Ouwehand AC, Salminen S (2003) Displacement of bacterial pathogens from mucus and Caco-2 cell surface by lactobacilli. J Med Microbiol 52:925–930
Marnila P, Rokka S, Rehnberg-Laiho L, Karkkainen P, Kosunen TU, Rautelin H, Hanninen ML, Syvaoja EL, Korhonen H (2003) Prevention and suppression of Helicobacter felis infection in mice using colostral preparation with specific antibodies. Helicobacter 8:192–201
Marteau PR, de Vrese M, Cellier CJ, Schrezenmeir J (2001) Protection from gastrointestinal diseases with the use of probiotics. Am J Clin Nutr 73 (Suppl 2):430–436
Ouwehand AC, Salminen SJ (1998) The health effects of cultured milk products with viable and non-viable bacteria. Int dairy J 8:749–758
Pakkanen R, Aalto J (1997) Growth factors and antimicrobial factors of bovine colostrum. Int Dairy J 7:285–297
Petschow BW, Talbott RD (1994) Reduction in virus-neutralizing activity of a bovine colostrum immunoglobulin concentrate by gastric acid and digestive enzymes. J Pediatr Gastroenterol Nutr 19:228–235
Petterson L, Graf W, Alm L, Lindwall S, Stroemberg A (1983) Survival of L. acidophilus NCDO 1748 in the human gastrointestinal tract. 1. Incubation with gastric juice in vitro. Symp Swed Nutr Found 15:123–125
Roos N, Mahe S, Benamouzig R, Sick H, Rautureau J, Tome D (1995) 15N-labeled immunoglobulins from bovine colostrum are partially resistant to digestion in human intestine. J Nutr 125:1238–1244
Ruiz LP (1994) Antibodies from milk for the prevention and treatment of diarrhea disease. Processing of the IDF seminar indigenous antimicrobial agents of milk-Recent developments. Int Dairy Fed Spec Issue 9404:108–121
Salminen S, Bouley C, Boutron-Ruanlt MC, Cummings JH, Franck A, Gibson GR, Isolauri E, Moreau MC, Roberfroid M, Rowland I (1998) Functional food science and gastrointestinal physiology and function. Br J Nutr 80 (suppl 2):147–171
Malin M, Verronen P, Korhonen H, Syvaja EL, Salminen S, Mykkien H, Arvilommi H, Eerola E, Isolauri E (1997) Dietary therapy with Lactobacillus GG, bovine colostrum or bovine immune colostrum in patients with juvenile chronic arthritis: evaluation of effect on gut defense mechanisms. Inflammopharmacology 5:219–236
Silva M, Jacobus NV, Deneke C, Gorbach SL (1987) Antimicrobial substance from a human L. strain. Antimicrob Agents Chemother 31:1231–1233
Viljanen M, Savilahti E, Haahtela T, Juntunen-Backman K, Korpela R, Poussa T, Tuure T, Kuitunen M (2005) Probiotics in the treatment of atopic eczema/dermatitis syndrome in infants: a double-blind placebo-controlled trial. Allergy 60:494–500
Warny M, Fatimi A, Bostwick EF, Laine DC, Lebel F, LaMont JT, Pothoulakis C, Kelly CP (1999) Bovine immunoglobulin concentrate—Clostridium difficile retains C. difficile toxin neutralising activity after passage through the human stomach and small intestine. Gut 44:212–217
Wei H, Marnila P, Korhonen H (2002a) Effects of anti-caries antibodies on Lactobacillus GG in its fermentation and storage periods. Biomed Environ Sci 15:153–165
Wei H, Loimaranta V, Tenovuo J, Rokka S, Syvaoja EL, Korhonen H, Joutsjoki V, Marnila P (2002b) Stability and activity of specific antibodies against Streptococcus mutans and Streptococcus sobrinus in bovine milk fermented with L. rhamnosus strain GG or treated at ultra-high temperature. Oral Microbiol Immunol 17:9–15
Acknowledgments
This research project was supported by the Chinese Scholar Committee (CSC) and Jiangxi Provincial Department of Science and Technology.
Author information
Authors and Affiliations
Corresponding author
Additional information
An erratum to this article is available at https://doi.org/10.1007/s00253-007-0965-1.
Rights and permissions
About this article
Cite this article
Hua, W., Yang, X., Yonghua, X. et al. Synergistic antidigestion effect of Lactobacillus rhamnosus and bovine colostrums in simulated gastrointestinal tract (in vitro). Appl Microbiol Biotechnol 75, 619–626 (2007). https://doi.org/10.1007/s00253-007-0861-8
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00253-007-0861-8