Skip to main content

Advertisement

Springer Nature Link
Log in

Expression of citrate permease gene of plasmid pCM1 isolated from Lactococcus lactis subsp. lactis biovar diacetylactis NIAI N-7 in Lactobacillus casei L-49-4

  • Applied Genetics and Molecular Biotechnology
  • Published:
Applied Microbiology and Biotechnology Aims and scope Submit manuscript

Abstract

Recombinant vector pJLECit (8,232 bp) was constructed using citrate permease gene contained in the 3,919-bp fragment of plasmid pCM1 (8,280 bp) isolated from Lactococcus lactis subsp. lactis biovar diacetylactis NIAI N-7, repA and ori from pLU1, and pMB1 ori and the erythromycin resistance gene from pJIR418. Lactobacillus casei L-49-4 (plasmid-free mutant of strain L-49) harboring the constructed pJLECit converted citrate into diacetyl/acetoin. Citrate uptake rate of resting cells was the highest at pH 5.5 and 10 mM citrate concentration. Diacetyl formation activity by the cell-free extracts of Lb. casei L-49-4 (pJLECit) grown in de Man–Rogosa–Sharpe (MRS) broth was higher than that of cells grown in MRS broth without citrate. On the other hand, diacetyl reductase activity of cells grown in MRS broth was lower than that of cells grown in MRS broth without citrate.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Subscribe and save

Springer+ Basic
$34.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or eBook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7

Similar content being viewed by others

Explore related subjects

Discover the latest articles and news from researchers in related subjects, suggested using machine learning.

References

  • An HY, Miyamoto T (2006a) Cloning and sequencing of plasmid pLC494 isolated from human intestinal Lactobacillus casei: construction of an Escherichia coli-Lactobacillus shuttle vector. Plasmid 55:128–134

    Article  CAS  Google Scholar 

  • An HY, Miyamoto T (2006b) Electrotransformation of Lactobacillus casei L-49-4 using a shuttle vector pJLE121 constructed for Escherichia coli and lactobacilli. Milk Sci 55:15–21

    CAS  Google Scholar 

  • Anderson DG, McKay LL (1983) Simple and rapid method for isolating large plasmid DNA from lactic streptococci. Appl Environ Microbiol 46:549–552

    Article  CAS  Google Scholar 

  • Bandell M, Lhotte ME, Marty-Teysset C, Veyrat A, Prevost H, Dartois V, Divies C, Konings WN, Lolkema JS (1998) Mechanism of the citrate transporters in carbohydrate and citrate co-metabolism in Lactococcus and Leuconostoc species. Appl Environ Microbiol 64:1594–1600

    Article  CAS  Google Scholar 

  • Bringel F, Frey L, Hubert JC (1989) Characterization, cloning, curing, and distribution in lactic acid bacteria of pLP1, a plasmid from Lactobacillusplantarum CCM 1904 and its use in shuttle vector construction. Plasmid 22:193–202

    Article  CAS  Google Scholar 

  • de Figueroa RM, Benito de Cardenas IL, Sesma F, Alvarez F, de Ruiz Holgado AP, Oliver G (1996) Inducible transport of citrate in Lactobacillusrhamnosus ATCC 7469. J Appl Bacteriol 81:348–354

    PubMed  Google Scholar 

  • Drider D, Bekal S, Prevost H (2004) Genetic organization and expression of citrate permease in lactic acid bacteria. Genet Mol Res 3:273–281

    CAS  PubMed  Google Scholar 

  • García-Quintáns N, Magni C, de Mendoza D, López P (1998) The citrate transport system of Lactococcus lactis subsp. lactis biovar diacetylactis is induced by acid stress. Appl Environ Microbiol 64:850–857

    Article  Google Scholar 

  • Gosalbes MJ, Monedero V, Pérez-Martínez G (1999) Elements involved in catabolite repression and substrate induction of the lactose operon in Lactobacillus casei. J Bacteriol 131:3928–3934

    Article  Google Scholar 

  • Gosalbes MJ, Esteban CD, Galan JL, Pérez-Martínez G (2000) Integrative food-grade expression system based on the lactose regulon of Lactobacillus casei. Appl Environ Microbiol 66:4822–4828

    Article  CAS  Google Scholar 

  • Hugenholtz J, Perdon L, Abee T (1993) Growth and energy generation by Lactococcus lactis subsp. lactis biovar diacetylactis during citrate metabolism. Appl Environ Microbiol 59:4216–4222

    Article  CAS  Google Scholar 

  • Jernejc K, Legisa M (2002) The influence of metal ions on malic enzyme activity and lipid synthesis in Aspergillus niger. FEMS Microbiol Lett 217:185–190

    Article  CAS  Google Scholar 

  • Kaneko T, Suzuki H, Takahashi T (1986) Diacetyl formation and degradation by Streptococcus lactis subsp. diacetylactis 3022. Agric Biol Chem 50:2639–2641

    CAS  Google Scholar 

  • Kempler GM, McKay LL (1980) Improved medium for detection of Streptococcus lactis subsp. diacetylactis. Appl Environ Microbiol 39:926–927

    Article  CAS  Google Scholar 

  • Kennes C, Dubourguier HC, Albagnac G, Nyns EJ (1991) Citrate metabolism by Lactobacillus plantarum isolated from orange juice. J Appl Bacteriol 70:380–384

    Article  CAS  Google Scholar 

  • Leenhouts K, Bolhuis A, Venema G, Kok J (1998) Construction of a food-grade multiple-copy integration system for Lactococcus lactis. Appl Microbiol Biotechnol 49:417–423

    Article  CAS  Google Scholar 

  • Lopez de Felipe F, Magni C, de Mendoza D, Lopez P (1995) Citrate utilization gene cluster of the Lactococcus lactis biovar diacetylactis: organization and regulation of expression. Mol Gen Genet 10:590–599

    Article  Google Scholar 

  • Maassen CBM, Laman JD, Heijne den Bak-Glashouwer MJ, Tielen FJ, van Holten-Neelen JCPA, Hoogteijling L, Antonissen C, Leer RJ, Pouwels PH, Boersma WJA, Shaw DM (1999) Instruments for oral disease-intervention strategies: recombinant Lactobacillus casei expressing tetanus toxin fragment C for vaccination or myelin proteins for oral tolerance induction in multiple sclerosis. Vaccine 17:2117–2128

    Article  CAS  Google Scholar 

  • Marjo JCS, Jeroen H (1991) Citrate fermentation by Lactococcus and Leuconostoc spp. Appl Environ Microbiol 57:3535–3540

    Article  Google Scholar 

  • Mauricio M, Christian M, Paloma L, Diego MD (2000) Transcriptional control of the citrate-inducible citMCDEFGRP operon; encoding genes involved in citrate fermentation in Leuconostoc paramesenteroides. J Bacteriol 182:3904–3912

    Article  Google Scholar 

  • Nakamura S, Tagawa Y, Miyamoto T, Kataoka K (1992) Plasmid profiles of lactose-, citrate- and proteinase-negative mutants of Lactococcus lactis subsp. lactis biovar diacetylactis strain N-7. Milchwissenschaft 47:358–361

    CAS  Google Scholar 

  • Palles T, Beresford T, Condon S, Cogan TM (1998) Citrate metabolism in Lactobacillus casei and Lactobacillus plantarum. J Appl Microbiol 85:147–154

    Article  CAS  Google Scholar 

  • Rea MC, Cogan TM (2003) Glucose prevents citrate metabolism by enterococci. Int J Food Microbiol 88:201–206

    Article  CAS  Google Scholar 

  • Sambrook J, Fritsch EF, Maniais T (1989) Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY

    Google Scholar 

  • Sewaki T, Miyamoto T (2001) Functional property and application to strain improvement of plasmid DNAs in the genus Lactobacillus. Milk Sci 50:151–156

    CAS  Google Scholar 

  • Sloan J, Warner TA, Scott PT, Bannam TL, Berryman DI, Rood JI (1992) Construction of a sequenced Clostridium perfringens–Esherichia coli shuttle plasmid. Plasmid 27:207–211

    Article  CAS  Google Scholar 

  • Takala TM, Saris PEJ (2002) A food-grade cloning vector for lactic acid bacteria based on the nisin immunity gene nisI. Appl Microbiol Biotechnol 59:467–471

    Article  CAS  Google Scholar 

  • Westerfeld WW (1945) A colorimetric determination of blood acetoin. J Biol Chem 161:495–502

    CAS  Google Scholar 

Download references

Acknowledgments

Part of this study was supported by the regional innovative consortium project of the Ministry of Economy, Trade and Industry, Japan.

Author information

Authors and Affiliations

  1. The Graduate School of Natural Science and Technology, Okayama University, Tsushima, Okayama, 700-8530, Japan

    Hwa-Yong An, Harutoshi Tsuda & Taku Miyamoto

Authors
  1. Hwa-Yong An
    View author publications

    Search author on:PubMed Google Scholar

  2. Harutoshi Tsuda
    View author publications

    Search author on:PubMed Google Scholar

  3. Taku Miyamoto
    View author publications

    Search author on:PubMed Google Scholar

Corresponding author

Correspondence to Taku Miyamoto.

Rights and permissions

Reprints and permissions

About this article

Cite this article

An, HY., Tsuda, H. & Miyamoto, T. Expression of citrate permease gene of plasmid pCM1 isolated from Lactococcus lactis subsp. lactis biovar diacetylactis NIAI N-7 in Lactobacillus casei L-49-4. Appl Microbiol Biotechnol 74, 609–616 (2007). https://doi.org/10.1007/s00253-006-0702-1

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00253-006-0702-1

Keywords

Profiles

  1. Harutoshi Tsuda View author profile