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Optimization of critical medium components using response surface methodology for biomass and extracellular polysaccharide production by Agaricus blazei

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Abstract

Response surface methodology (RSM) was applied to optimize the critical medium ingredients of Agaricus blazei. A three-level Box–Behnken factorial design was employed to determine the maximum biomass and extracellular polysaccharide (EPS) yields at optimum levels for glucose, yeast extract (YE), and peptone. A mathematical model was then developed to show the effect of each medium composition and its interactions on the production of mycelial biomass and EPS. The model predicted the maximum biomass yield of 10.86 g/l that appeared at glucose, YE, peptone of 26.3, 6.84, and 6.62 g/l, respectively, while a maximum EPS yield of 348.4 mg/l appeared at glucose, YE, peptone of 28.4, 4.96, 5.60 g/l, respectively. These predicted values were also verified by validation experiments. The excellent correlation between predicted and measured values of each model justifies the validity of both the response models. The results of bioreactor fermentation also show that the optimized culture medium enhanced both biomass (13.91 ± 0.71 g/l) and EPS (363 ± 4.1 mg/l) production by Agaricus blazei in a large-scale fermentation process.

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References

  • Box GP, Behnken DW (1960) Some new three level design for the study of quantitative variables. Technometrics 2:456–475

    Article  Google Scholar 

  • Ebina T, Fujiyama Y (1998) Antitumor effect of a peptide-glucan preparation extracted from Agaricus blazei in a double grafted tumor system in mice. Biotherapy 11:259–265

    Article  CAS  Google Scholar 

  • Fujimiya Y, Suzuki Y, Oshiman K, Kobori H, Moriguchi K, Nakashima H, Matumoto Y, Takahara S, Ebina T, Katakura R (1998) Selective tumoricidal effect of soluble proteoglucan extracted from the basidiomycete, Agaricus blazei Murill, mediated via natural killer cell activation and apoptosis. Cancer Immunol Immunother 46:147–159

    Article  CAS  Google Scholar 

  • Hsieh CY, Hsu TH, Yang FC (2005) Production of polysaccharides of Ganoderma lucidum (CCRC36021) by reusing thin stillage. Process Biochem 40:909–916

    Article  CAS  Google Scholar 

  • Ishihara T (1999) Polysaccharide of Agaricus blazei Murill. In: Tanimura A (ed) Handbook of active substances in plant resources, 2nd edn. Science Forum, Tokyo, pp 348–350

    Google Scholar 

  • Kawagoe M, Nagaoka Y, Araki M, Yamagami K, Naoe K, Noda H (2004) Submerged culture of Agaricus blazei mycelium in a bubble column fermentor. J Chem Eng Japan 37:1056–1061

    Article  CAS  Google Scholar 

  • Kim HH, Na JG, Chang YK, Chun GT, Lee SJ, Jeong YH (2004) Optimization of submerged culture conditions for mycelial growth and exopolysaccharides production by Agaricus blazei. J Microbiol Biotechnol 14:944–951

    CAS  Google Scholar 

  • Liu GQ, Wang XL (2006) Submerged fermentation of higher fungus Agaricus blazei for production of biomass and extracellular polysaccharide. J Anhui Agric Sci 45:1645–1649

    Google Scholar 

  • Mao XB, Eksriwong T, Chauvatcharin S, Zhong JJ (2005) Optimization of carbon source and carbon/nitrogen ratio for cordycepin production by submerged cultivation of medicinal mushroom Cordyceps militaris. Process Biochem 40:1667–1672

    Article  CAS  Google Scholar 

  • Miller GL (1959) Use of dinitrosalicylic acid reagent for determination of reducing sugars. Anal Chem 31:426–428

    Article  CAS  Google Scholar 

  • Mizuno T (1995) Bioactive biomolecules of mushrooms—food, function and medicinal effects of mushroom fungi. Food Res Int 11:7–21

    CAS  Google Scholar 

  • Mizuno T (2002) Medicinal properties and clinical effects of culinary-medicinal mushroom Agaricus blazei Murrill (Agaricomycetideae) (review). Int J Med Mushrooms 4:299–312

    Article  Google Scholar 

  • Mizuno M, Morimoto M, Minato K, Tschida H (1998) Polysaccharides from Agaricus blazei stimulate lymphocyte T-cell subsets in mice. Biosci Biotechnol Biochem 62:434–437

    Article  CAS  Google Scholar 

  • Nakajima A, Ishida T, Koga M, Takeuchi T, Mazda O, Takeuchi M (2002) Effect of hot water extract from Agaricus blazei Murill on antibody-producing cells in mice. Int Immunopharmacol 2:1205–1211

    Article  CAS  Google Scholar 

  • Prapulla SG, Jacob S, Chand N, Rajalakshmi D, Karanth NG (1992) Maximization of lipid production by Rhodotorula gracilis CFR-1 using response surface methodology. Biotechnol Bioeng 40:965–969

    Article  CAS  Google Scholar 

Download references

Acknowledgement

The authors would like to thank Mr. Jian-Nan Chen for his helpful assistance in the determination of polysaccharide content.

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Correspondence to Gao-Qiang Liu.

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Liu, GQ., Wang, XL. Optimization of critical medium components using response surface methodology for biomass and extracellular polysaccharide production by Agaricus blazei . Appl Microbiol Biotechnol 74, 78–83 (2007). https://doi.org/10.1007/s00253-006-0661-6

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  • DOI: https://doi.org/10.1007/s00253-006-0661-6

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