Hypoglycemic activity of the fungi Cordyceps militaris, Cordyceps sinensis, Tricholoma mongolicum, and Omphalia lapidescens in streptozotocin-induced diabetic rats
Crude extracts were prepared from fruiting bodies and mycelia of the medicinal fungus Cordyceps militaris, and a polysaccharide-enriched fraction was obtained after extraction with hot water and ethanol precipitation. Polysaccharide-enriched fractions were similarly prepared from Cordyceps sinensis, Omphalia lapidescens, and Tricholoma mongolicum. The various aforementioned preparations were orally administered into different groups of adult rats 24 h before an intraperitoneal injection of streptozotocin (40 mg/kg body weight), and subsequently daily for another 4 days. The dosage used was 10 mg/kg body weight for polysaccharide-enriched preparations and 100 mg/kg body weight for crude extracts. Control rats received distilled water instead of crude extract or polysaccharide-enriched preparation. It was found in the control rats that plasma glucose level rose from about 90 mg/dl before streptozotocin injection to levels that were maintained at about 300 mg/dl postinjection. All preparations produced hypoglycemic effects. C. militaris polysaccharide-enriched fraction displayed a more prominent effect than that of C. sinensis polysaccharide-enriched fraction which in turn was more potent than that of O. lapidescens and T. mongolicum polysaccharide-enriched fractions. The hypoglycemic effect of C. militaris polysaccharide-enriched fraction was dose-dependent.
- Kiho T, Hui J, Yamane A, Ukai S (1993) Polysaccharides in fungi. XXXII. Hypoglycemic activity and chemical properties of a polysaccharide from the cultural mycelium of Cordyceps sinensis. Biol Pharm Bull 16:291–1293Google Scholar
- Liu YK, Shen W (2003) Inhibitive effect of Cordyceps sinensis on experimental hepatic fibrosis and its possible mechanism. Word J Gastroenterol 9:529–533Google Scholar
- Tsai CH, Stern A, Chiou JF, Chern CL, Liu TZ (2001) Rapid and specific detection of hydroxyl radical using an ultraweak chemiluminescence analyzer and a low-level chemiluminescence emitter: application to hydroxyl radical-scavenging ability of aqueous extracts of food constituents. J Agric Food Chem 49:2137–2141CrossRefPubMedGoogle Scholar
- Zhang W, Yang J, Chen J, Hou Y, Han X (2004a) Immunomodulatory and antitumor effects of exopolysaccharide fraction (EPSF) from a cultivated Cordyceps sinensis fungus on tumor-bearing mice. Biotechnol Appl Biochem 42:9–15Google Scholar