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Decreased incidence of disease caused by Sclerotinia sclerotiorum and improved plant vigor of oilseed rape with Bacillus subtilis Tu-100

Applied Microbiology and Biotechnology volume 68, pages 802–807 (2005)Cite this article

Abstract

Sclerotinia sclerotiorum causes serious yield losses in oilseed crops worldwide. Bacillus subtilis Tu-100 significantly reduced (P≤0.05) the incidence of disease caused by S. sclerotiorum on oilseed rape at harvest in two trials conducted in fields artificially infested with this pathogen. Mean plant dry weight was significantly greater (P≤0.05) and mean plant length was significantly greater (P≤0.07) at the seven-true-leaf stage with the Tu-100 treatment than with the control. Mean seed yield per 120 plants at harvest was significantly greater (P≤0.05) in the second field trial with treatments containing isolate Tu-100. B. subtilis Tu-100 also promoted the growth of hydroponically grown oilseed rape. Plants were approximately 15% greater in dry weight (P≤0.0001) and 6% greater in length (P≤0.0025) when grown in the presence of isolate Tu-100 in Hoagland’s solution, compared with the noninoculated control. In gnotobiotic studies, the lacZ-tagged strain B. subtilis Tu-100(pUC18) was detected within all roots of oilseed rape. Isolate Tu-100 did not persist in the ectorhizosphere of oilseed rape. Populations of this isolate decreased from 8.5×108 colony-forming units (CFU) per seed to approximately 102 CFU in the plant ectorhizosphere within 30 days of sowing in autoclaved soil.

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Acknowledgement

This project was supported by the National Natural Science Foundation of China (Project number 39870043).

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Affiliations

  1. Key Laboratory for Genetic Improvement of Oil Crops, Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan, 430062, People’s Republic of China

    Xiaojia Hu, Mulan Jiang & Yinbo Zhang

  2. Sustainable Agricultural Systems Laboratory, Henry A. Wallace Beltsville Agricultural Research Center, United States Department of Agriculture–Agricultural Research Service, Beltsville, MD 20705, USA

    Daniel P. Roberts

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  1. Xiaojia Hu
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  2. Daniel P. Roberts
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  3. Mulan Jiang
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  4. Yinbo Zhang
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Correspondence to Daniel P. Roberts.

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Hu, X., Roberts, D.P., Jiang, M. et al. Decreased incidence of disease caused by Sclerotinia sclerotiorum and improved plant vigor of oilseed rape with Bacillus subtilis Tu-100. Appl Microbiol Biotechnol 68, 802–807 (2005). https://doi.org/10.1007/s00253-005-1938-x

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  • DOI: https://doi.org/10.1007/s00253-005-1938-x

Keywords

  • Oilseed Rape
  • Potato Dextrose Agar Plate
  • Lateral Root Formation
  • Bacterial Endophyte
  • Autoclave Soil