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Aluminum- and mild steel-binding peptides from phage display

Applied Microbiology and Biotechnology volume 68, pages 505–509 (2005)Cite this article

Abstract

Using a phage library displaying random peptides of 12 amino acids on its surface, several peptides were found that bind to aluminum and mild steel. Like other metal-binding peptides, no obvious consensus motif has been found for these peptides. However, most of them are rich in hydroxyl-containing amino acids, serine or threonine, or contain histidine. For the aluminum-binding peptides, peptides with a higher number of hydroxyl-containing amino acids bind to the aluminum surface more tightly. For example, Val-Pro-Ser-Ser-Gly-Pro-Gln-Asp-Thr-Arg-Thr-Thr, which contains five hydroxyl-containing amino acid residues, was selected four-fold more frequently than a peptide containing only one serine, suggesting an important role for the hydroxyl-containing amino acids in the metal–peptide interaction.

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References

  • Bae W, Chen W, Mulchandani A, Mehra RK (2000) Enhanced bioaccumulation of heavy metals by bacterial cells displaying synthetic phytochelatins. Biotechnol Bioeng 70:518–524

    Article  PubMed  CAS  Google Scholar 

  • Beitle RR, Ataai MM (1993) One-step purification of a model periplasmic protein from inclusion bodies by its fusion to an effective metal-binding peptide. Biotechnol Prog 9:64–69

    Article  PubMed  CAS  Google Scholar 

  • Brown S (1997) Metal-recognition by repeating polypeptides. Nat Biotechnol 15:269–272

    Article  PubMed  CAS  Google Scholar 

  • Canada KA, Iwashita S, Shim H, Wood TK (2002) Directed evolution of toluene ortho-monooxygenase for enhanced 1-naphthol synthesis and chlorinated ethene degradation. J Bacteriol 184:344–349

    PubMed  Article  CAS  Google Scholar 

  • DeSilva TM, Veglia G, Porcelli F, Prantner AM, Opella SJ (2002) Selectivity in heavy metal-binding to peptides and proteins. Biopolymers 64:189–197

    Article  PubMed  CAS  Google Scholar 

  • Flynn CE, Lee S-W, Peelle BR, Belcher AM (2003a) Viruses as vehicles for growth, organization and assembly of materials. Acta Mater 51:5867–5880

    Article  CAS  Google Scholar 

  • Flynn CE, Mao C, Hayhurst A, Williams JL, Georgiou G, Iverson B, Belcher AM (2003b) Synthesis and organization of nanoscale II–VI semiconductor materials using evolved peptide specificity and viral capsid assembly. J Mater Chem 13:2414–2421

    Article  CAS  Google Scholar 

  • Hoogenboom HR, Chames P (2000) Natural and designer binding sites made by phage display technology. Immunol Today 21:371–378

    Article  PubMed  CAS  Google Scholar 

  • Jayaraman A, Cheng ET, Earthman JC, Wood TK (1997a) Axenic aerobic biofilms inhibit corrosion of SAE 1018 steel through oxygen depletion. Appl Microbiol Biotechnol 48:11–17

    Article  PubMed  CAS  Google Scholar 

  • Jayaraman A, Cheng ET, Earthman JC, Wood TK (1997b) Importance of biofilm formation for corrosion inhibition of SAE 1018 steel by axenic aerobic biofilms. J Ind Microbiol Biotechnol 18:396–401

    Article  PubMed  CAS  Google Scholar 

  • Jayaraman A, Earthman JC, Wood TK (1997c) Corrosion inhibition by aerobic biofilms on SAE 1018 steel. Appl Microbiol Biotechnol 47:62–68

    Article  CAS  Google Scholar 

  • Jayaraman A, Hallock PJ, Carson RM, Lee C-C, Mansfeld FB, Wood TK (1999a) Inhibiting sulfate-reducing bacteria in biofilms on steel with antimicrobial peptides generated in situ. Appl Microbiol Biotechnol 52:267–275

    Article  PubMed  CAS  Google Scholar 

  • Jayaraman A, Mansfeld FB, Wood TK (1999b) Inhibiting sulfate-reducing bacteria in biofilms by expressing the antimicrobial peptides indolicidin and bactenecin. J Ind Microbiol Biotechnol 22:167–175

    Article  CAS  Google Scholar 

  • Jayaraman A, Örnek D, Duarte DA, Lee C-C, Mansfeld FB, Wood TK (1999c) Axenic aerobic biofilms inhibit corrosion of copper and aluminum. Appl Microbiol Biotechnol 52:787–790

    Article  PubMed  CAS  Google Scholar 

  • Koch GH, Brongers MPH, Thompson NG, Virmani YP, Payer JH (2001) Corrosion costs and preventive strategies in the United States. (Report FHWA-RD-01-156) FHWA, Washington, D.C.

  • Kotrba P, Delečková L, De Lorenzo V, Ruml T (1999) Enhanced bioaccumulation of heavy metal ions by bacterial cells due to surface display of short metal binding peptides. Appl Environ Microbiol 65:1092–1098

    PubMed  CAS  Google Scholar 

  • Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.

    Google Scholar 

  • Manoutcharian K, Gevorkian G, Cano A, Almagro JC (2001) Phage displayed biomolecules as preventive and therapeutic agents. Curr Pharm Biotechnol 2:217–223

    Article  PubMed  CAS  Google Scholar 

  • Mao C, Flynn CE, Hayhurst A, Sweeney R, Qi J, Georgiou G, Iverson B, Belcher AM (2003) Viral assembly of oriented quantum dot nanowires. Proc Natl Acad Sci USA 100:6946–6951

    Article  PubMed  CAS  Google Scholar 

  • Matsubara T, Hiura Y, Kawahito O, Yasuzawa M, Kawashiro K (2003) Selection of novel structural zinc sites from a random peptide library. FEBS Lett 555:317–321

    Article  PubMed  CAS  Google Scholar 

  • Matzapetakis M, Farrer BT, Weng T-C, Hemmingsen L, Penner-Hahn JE, Pecoraro VL (2002) Comparison of the binding of cadmium(II), mercury(II), and arsenic(III) to the de novo designed peptides TRI L12C and TRI L16C. J Am Chem Soc 124:8042–8054

    Article  PubMed  CAS  Google Scholar 

  • Naik RR, Stringer SJ, Agarwal G, Jones SE, Stone MO (2002) Biomimetic synthesis and patterning of silver nanoparticles. Nat Mater 1:169–172

    Article  PubMed  CAS  Google Scholar 

  • Nam KT, Peelle BR, Lee S-W, Belcher AM (2004) Genetically driven assembly of nanorings based on the M13 virus. Nano Lett 4:23–27

    Article  CAS  Google Scholar 

  • Örnek D, Jayaraman A, Syrett BC, Hsu C-H, Mansfeld FB, Wood TK (2002a) Pitting corrosion inhibition of aluminum 2024 by Bacillus biofilms secreting polyaspartate or γ-polyglutamate. Appl Microbiol Biotechnol 58:651–657

    Article  PubMed  CAS  Google Scholar 

  • Örnek D, Wood TK, Hsu CH, Mansfeld F (2002b) Corrosion control using regenerative biofilms (CCURB) on brass in different media. Corros Sci 44:2291–2302

    Article  Google Scholar 

  • Patwardhan AV, Goud GN, Koepsel RR, Ataai MM (1997) Selection of optimum affinity tags from a phage-displayed peptide library. Application to immobilized copper(II) affinity chromatography. J Chromatogr A 787:91–100

    Article  PubMed  CAS  Google Scholar 

  • Pazirandeh M, Wells BM, Ryan RL (1998) Development of bacterium-based heavy metal biosorbents: enhanced uptake of cadmium and mercury by Escherichia coli expressing a metal binding motif. Appl Environ Microbiol 64:4068–4072

    PubMed  CAS  Google Scholar 

  • Petrenko VA, Vodyanoy VJ (2003) Phage display for detection of biological threat agents. J Microbiol Methods 53:253–262

    Article  PubMed  CAS  Google Scholar 

  • Potekhina JS, Sherisheva NG, Povetkina LP, Pospelov AP, Rakitina TA, Warnecke F, Gottschalk G (1999) Role of microorganisms in corrosion inhibition of metals in aquatic habitats. Appl Microbiol Biotechnol 52:639–646

    Article  CAS  Google Scholar 

  • Samuelson P, Wernérus H, Svedberg M, Ståhl S (2000) Staphylococcal surface display of metal-binding polyhistidyl peptides. Appl Environ Microbiol 66:1243–1248

    Article  PubMed  CAS  Google Scholar 

  • Satoh M, Karaki E, Kakehashi M, Okazaki E, Gotoh T, Oyama Y(1999) Heavy-metal induced changes in nonproteinaceous thiol levels and heavy-metal binding peptide in Tetraselmis tetrathele (Prasinophyceae). J Phycol 35:989–994

    Article  CAS  Google Scholar 

  • Scott JK, Smith GP (1990) Searching for peptide ligands with an epitope library. Science 249:386–390

    PubMed  Article  CAS  Google Scholar 

  • Smith GP (1985) Filamentous fusion phage: novel expression vectors that display cloned antigens on the virion surface. Science 228:1315–1317

    PubMed  Article  CAS  Google Scholar 

  • Sousa C, Kotrba P, Rumi T, Cebolla A, De Lorenzo V (1998) Metalloadsorption by Escherichia coli cells displaying yeast and mammalian metallothioneins anchored to the outer membrane protein LamB. J Bacteriol 180:2280–2284

    PubMed  CAS  Google Scholar 

  • Volz J, Bosch FU, Wunderlin M, Schuhmacher M, Melchers K, Bensch K, Steinhilber W, Schafer KP, Toth G, Penke B, Przybylski M (1998) Molecular characterization of metal-binding polypeptide domains by electrospray ionization mass spectrometry and metal chelate affinity chromatography. J Chromatogr A 800:29–37

    Article  PubMed  CAS  Google Scholar 

  • Whaley SR, English DS, Hu EL, Barbara PF, Belcher AM (2000) Selection of peptides with semiconductor binding specificity for directed nanocrystal assembly. Nature 405:665–668

    Article  PubMed  CAS  Google Scholar 

  • Wilson DR, Finlay BB (1998) Phage display: applications, innovations, and issues in phage and host biology. Can J Microbiol 44:313–329

    Article  PubMed  CAS  Google Scholar 

  • Xu Z, Lee SY (1999) Display of polyhistidine peptides on the Escherichia coli cell surface by using outer membrane protein C as an anchoring motif. Appl Environ Microbiol 65:5142–5147

    PubMed  CAS  Google Scholar 

  • Zuo R, Wood TK (2004) Inhibiting mild steel corrosion from sulfate-reducing and iron-oxidizing bacteria using gramicidin-S-producing biofilms. Appl Microbiol Biotechnol 65:747–753

    Article  PubMed  CAS  Google Scholar 

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Acknowledgements

This project was supported by the Electric Power Research Institute (contract WO8044-0).

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Author notes

  1. Dogan Örnek

    Present address: Genzyme Corporation, Cambridge, MA 02139, USA

Authors and Affiliations

  1. Departments of Chemical Engineering and Molecular and Cell Biology, University of Connecticut, Storrs, CT 06269-3222, USA

    Rongjun Zuo, Dogan Örnek & Thomas K. Wood

Authors
  1. Rongjun Zuo
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  2. Dogan Örnek
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  3. Thomas K. Wood
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Correspondence to Thomas K. Wood.

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Zuo, R., Örnek, D. & Wood, T.K. Aluminum- and mild steel-binding peptides from phage display. Appl Microbiol Biotechnol 68, 505–509 (2005). https://doi.org/10.1007/s00253-005-1922-5

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  • DOI: https://doi.org/10.1007/s00253-005-1922-5

Keywords

  • Mild Steel
  • Phage Display
  • Peptide Library
  • Phage Display Peptide Library
  • Random Peptide Library