Analysis of genetic diversity in Ganoderma population with a novel molecular marker SRAP
- 933 Downloads
Genetic marker technology designed to detect naturally occurring polymorphisms at the DNA level had become an invaluable and revolutionizing tool for both applied and basic studies of fungi. To eliminate the confusion on the taxonomy of Ganoderma strains, in this study, a collection of 31 accessions representative of morphotypes and some unclassified types was used for analyzing molecular diversity using a novel molecular marker sequence-related amplified polymorphism (SRAP). This collection included commercial cultivars and wild varieties that represented the great diversification of types from different countries and regions. The experimental results showed that 50 out of 95 combinations of primers turned out to be polymorphic, and 85 polymorphism bands were obtained using six combinations. Based on the appearances of markers, the genetic similarity coefficients were calculated, and genetic variations were observed (0∼1) among the 31 different Ganoderma strains. The group of Ganoderma lucidum showed significant differences from the group of Ganoderma sinense. Moreover, G. lucidum in China was also different from G. lucidum in Yugoslavia. At the same time, cluster analysis successfully categorized these 31 Ganoderma strains into five groups. These results revealed the genetic diversity of Ganoderma strains and their correlation with geographic environments. It also suggested SRAP marker could be used in the taxonomic analysis of fungi. To our knowledge, this is the first application of SRAP marker on the systematics of Ganoderma strains within basidiomycetes.
KeywordsGanoderma Genetic Diversity Analysis SRAP Marker Flammulina Velutipes TaKaRa Biotech
This work was financially supported by a grant from Science & Technology Department of Fujian Province, People’s Republic of China (2003EAT20021). We would like to sincerely thank Dr. Hu Zhu and Tianwen Wang of East China University of Science and Technology for their critical reading of the manuscript and useful suggestions.
- Flores Berrios EP, Alba Gonzalez JF, Arrizon Gavino JP, Romano P, Capece A, Gschaedler Mathis A (2005) The uses of AFLP for detecting DNA polymorphism, genotype identification and genetic diversity between yeasts isolated from Mexican agave-distilled beverages and from grape musts. Lett Appl Microbiol 41:147–152CrossRefPubMedGoogle Scholar
- Graham GC, Mayers P, Henry RJ (1994) A simplified method for the preparation of fungal genomic DNA for PCR and RAPD analysis. Biotechniques 16:49–50Google Scholar
- Jaccard P (1908) Nouvelles recherches surla distribution florale. Bull Soc Vaudoise Sci Nat 44:223–270Google Scholar
- Lin ZB (2001) Modern study on Ganoderma lucidum. Peking Medical Science University, BeijingGoogle Scholar
- Ryvarden L (1994) Can we trust morphology in Ganoderma. In: Buchanan PK, Hseu RS, Moncalvo JM (eds) Ganoderma: systematics, phytopathology and pharmacology. Proceedings of contributed symposium 59 A, B. Fifth International Mycological Congress, Vancouver, August 14-21, 1994, pp 19–24Google Scholar
- Sokol S, Kaldorf M, Bothe H (1999) Molecular characterization and taxonomic affinities of species of the white rot fungus Ganoderma. Z Naturforsch 54:314–318Google Scholar
- Su JM, Fu RH, Zhou JB, Zhang LH (2002) Applying practice of SPSS serial of statistics software: cluster analysis. Publishing House of Electronics Industry, BeijingGoogle Scholar
- Zeng FY, Zhang YZ (1996) Preparation of edible fungus DNA from polysaccharide-rich sample. Acta Edulis Fungi 3:13–17Google Scholar