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A recombinant Escherichia coli whole cell biocatalyst harboring a cytochrome P450cam monooxygenase system coupled with enzymatic cofactor regeneration

  • Applied Genetics and Molecular Biotechnology
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Abstract

A cytochrome P450cam monooxygenase (P450cam) system from the soil bacterium Pseudomonas putida requires electron transfer among three different proteins and a cofactor, nicotinamide adenine dinucleotide (NADH), for oxygenation of its natural substrate, camphor. Herein, we report a facile way to significantly enhance the catalytic efficiency of the P450cam system by the coupling of its native electron transfer system with enzymatic NADH regeneration catalyzed by glycerol dehydrogenase (GLD) in Escherichia coli whole cell biocatalysts. Recombinant E. coli harboring the P450cam system, but lacking GLD, exhibited little activity for camphor hydroxylation. In contrast, coexpression of GLD with the proteinaceous electron transfer components of P450cam resulted in about tenfold improvement in the substrate conversion, implying that the whole cell biocatalyst utilized molecular oxygen, endogenous NADH, and glycerol in the cell for catalysis. The addition of glycerol to the reaction media further promoted camphor hydroxylation, suggesting that exogenous glycerol is also available for GLD in the host cell and actively participates in the catalytic cycle. These results clearly show the utility of GLD towards functional reconstruction of the native P450cam system. The present approach may also be useful for E. coli whole cell biocatalysts with the other NADH-dependent oxygenases and oxidoreductases.

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Acknowledgement

This research was supported by the 21st Century Center of Excellence Program “Functional Innovation of Molecular Informatics” from the Ministry of Education, Culture, Sports, Science, and Technology of Japan (to M.G.).

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Authors and Affiliations

  1. Department of Applied Chemistry, Graduate School of Engineering, Kyushu University, 744 Motooka, Fukuoka, 819-0395, Japan

    Tsuyoshi Mouri, Junji Michizoe, Noriho Kamiya & Masahiro Goto

  2. Center for Future Chemistry, Kyushu University, 744 Motooka, Fukuoka, 819-0395, Japan

    Noriho Kamiya & Masahiro Goto

  3. Goto Nano-Bio Project in Momochi, Japan Science and Technology Agency, 3-8-34 Momochihama, Fukuoka, 814-0001, Japan

    Hirofumi Ichinose

Authors
  1. Tsuyoshi Mouri
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  2. Junji Michizoe
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  3. Hirofumi Ichinose
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  4. Noriho Kamiya
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  5. Masahiro Goto
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Corresponding author

Correspondence to Masahiro Goto.

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Mouri, T., Michizoe, J., Ichinose, H. et al. A recombinant Escherichia coli whole cell biocatalyst harboring a cytochrome P450cam monooxygenase system coupled with enzymatic cofactor regeneration. Appl Microbiol Biotechnol 72, 514–520 (2006). https://doi.org/10.1007/s00253-005-0289-y

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  • DOI: https://doi.org/10.1007/s00253-005-0289-y

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