Skip to main content
Log in

A novel membrane bioreactor enhanced by effective microorganisms for the treatment of domestic wastewater

  • Environmental Biotechnology
  • Published:
Applied Microbiology and Biotechnology Aims and scope Submit manuscript

Abstract

The activated sludge membrane bioreactor (MBR) has been shown to have some advantages for the processing and reclamation of domestic wastewater. We hypothesized that certain microorganisms, chosen for their abilities to decompose the chemical components of raw sewage, would, when coupled with the MBR, significantly improve the stability and efficiency of this system. We selected environmental bacterial strains which oxidize ammonia and nitrites and produce protease, amylase, and cellulase for the development and testing of a novel biologically enhanced MBR (eMBR). We compared the eMBR with the activated sludge MBR. With the eMBR, the average values of effluent quality were: chemical oxygen demand (COD), 40 mg/l(average efficiency of removal 90.0%); and NH4 +–N, 0.66 mg/l(average efficiency of removal 99.4%). Effluent qualities met the standard and were stable during the entire 90 days of this study. For the activated sludge MBR, the COD removal rate was 91.7%, and the NH4 +–N removal (94.8%) was less than that of the eMBR. Start-up time for the eMBR was only 24–48 h, much shorter than the 7–8 days required to initiate function of the standard MBR. The biomass concentrations of total heterotrophic bacteria and autotrophic bacteria in the eMBR did not fluctuate significantly during the course of the study. Various kinds of microorganisms will establish an ecological balance in the reactor. Compared with the activated sludge MBR, the eMBR not only produced an excellent and stable quality of effluent but also resulted in a shorter time to start-up and significantly improved the efficiency of NH4 +–N removal.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Subscribe and save

Springer+ Basic
$34.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or eBook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7

Similar content being viewed by others

Explore related subjects

Discover the latest articles and news from researchers in related subjects, suggested using machine learning.

References

  • Bakir HA (2001) Sustainable wastewater management for small communities in the Middle East and North Africa. J Environ Manag 61:319–328

    Article  CAS  Google Scholar 

  • Chen SM, Zheng FS (1985) Experimentation of aquatic microbiology. Ocean, Beijing, China

    Google Scholar 

  • Chiemchaisri C, Wong YK, Urase T, Yamamoto K (1992) Organic stabilization and nitrogen removal in membarne separation bioreactor for domestic wastewater treatment. Water Sci Technol 25:231–240

    Article  CAS  Google Scholar 

  • Cicek N (2003) A review of membrane bioreactors and their potential application in the treatment of agricultural wastewater. Can Biosyst Eng 45:637–649

    Google Scholar 

  • Cicek N, Franco JP, Suidan MT, Urbain V (1998) Using a membrane bioreactor to reclaim wastewater. J AWWA 90:105–113

    Article  CAS  Google Scholar 

  • Clesceri LS, Greenberg AE, Eaton AD (eds) (1998) Standard methods for the examination of water and wastewater, 20th edn. APHA, Washington, DC

    Google Scholar 

  • DiGiano FA, Andreottola G, Adham S, Buckley C, Cornel P, Daigger GT, Fane AG, Galil N, Jacangelo J, Pollice A, Rittmann BE, Rozzi A, Stephenson T, Ojang Z (2004) Membrane bioreactor technology. http://www.scienceinafrica.co.za/2004/june/membrane.htm

  • General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China (2002) Water quality standard for urban miscellaneous water consumption (GB/T 18920-2002). Beijing

  • Giraud E, Brauman A, Keleke S, Lelong B, Raimbault M (1991) Isolation and physiological study of an amylolytic strain of Lactobacillus plantarum. Appl Microbiol Biotechnol 36:379–383

    CAS  Google Scholar 

  • Hankin L, Anagnostakis SL (1977) Solid media containing carboxymethylcellulose to detect Cx cellulase activity of microorganisms. J Gen Microbiol 98:109–115

    Article  CAS  Google Scholar 

  • Higa T (1991) Effective microorganisms: a biotechnology for mankind. In: Parr JF, Hornick SB, Whitman CE (eds) Proceedings of the first international conference on Kyusei nature farming. US Department of Agriculture, Washington, DC, USA, pp 8–14

    Google Scholar 

  • Higa T, Wididana GN (1991) The concept and theories of effective microorganisms. In: Parr JF, Hornick SB, Whitman CE (eds) Proceedings of the first international conference on Kyusei nature farming. US Department of Agriculture, Washington, DC, USA, pp 118–124

    Google Scholar 

  • Li XY, Chu HP (2003) Membrane bioreactor for the drinking water treatment of polluted surface water supplies. Water Res 37:4781–4791

    Article  CAS  Google Scholar 

  • Liu R, Huan X, Qian Y, Zhang MM (1999) The pilot study on treatment of domestic wastewater using by MBR. Water Wastewater 25:1–4

    CAS  Google Scholar 

  • Muller EB, Stouthamer AH, Verseveld HW, Eikelboom DH (1995) Aerobic domestic waste water treatment in a pilot plant with complete sludge retention by cross-flow filtration. Water Res 29:1179–1189

    Article  CAS  Google Scholar 

  • Olson GE (2004) Hunters Point Shipyard Decentralized Wastewater Treatment Study. Project no. WU-HT-01-34. Prepared for the National Decentralized Water Resources Capacity Development Project, Washington University, St. Louis, MO, by the San Francisco Public Utilities Commission, San Francisco, CA

  • Rosenberger S, Kruger U, Witzig R, Manz W, Szewzyk U, Kraume M (2002) Performance of a bioreactor with submerged membrane for aerobic treatment of municipal waste water. Water Res 36:413–420

    Article  CAS  Google Scholar 

  • State Environmental Protection Administration of China (2004) Report on the environmental conditions in China

  • Stephenson T, Judd S, Jefferson B, Brindle K (2000) Membrane bioreactors for wastewater treatment. IWA, London, pp 59–61

    Google Scholar 

  • Sundaravadivel M, Vigneswaran S (2001) Wastewater collection and treatment technologies for semi-urban areas of India: a case study. Water Sci Technol 43:329–336

    Article  CAS  Google Scholar 

  • Teather RM, Wood PJ (1982) Use of Congo red–polysaccharide interactions in enumeration and characterization of cellulolytic bacteria from bovine rumen. Appl Environ Microbiol 43:777–780

    Article  CAS  Google Scholar 

  • Ueda T, Hata K (1999) Domestic wastewater treatment by a submerged membrane bioreactor with gravitational filtration. Water Res 33:2888–2892

    Article  CAS  Google Scholar 

  • Van de Roest HF, Lawrence DP, Van Bentem AGN (2002) Membrane bioreactors for municipal wastewater treatment. STOWA Report. IWA, London

    Google Scholar 

  • Visvanathan C, Aim RB, Parameshwaran K (2000) Membrane separation bioreactor for wastewater treatment. Crit Rev Environ Sci Technol 30:1–48

    Article  CAS  Google Scholar 

  • Williams CM, Richter CS, MacKenzie JM, Shih JCH (1990) Isolation, identification and characterisation of a feather degrading bacterium. Appl Environ Microbiol 56:1509–1515

    Article  CAS  Google Scholar 

Download references

Acknowledgements

This work presents a part of the results of the Nation High Technology Research and Development Program of China (“863” Projects, 2002AA601240) and the key Project of Sci&Tech of Tianjin (No. 023180711).

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Jun-Wen Li.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Jin, M., Wang, XW., Gong, TS. et al. A novel membrane bioreactor enhanced by effective microorganisms for the treatment of domestic wastewater. Appl Microbiol Biotechnol 69, 229–235 (2005). https://doi.org/10.1007/s00253-005-0108-5

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00253-005-0108-5

Keywords