Abstract
The activated sludge membrane bioreactor (MBR) has been shown to have some advantages for the processing and reclamation of domestic wastewater. We hypothesized that certain microorganisms, chosen for their abilities to decompose the chemical components of raw sewage, would, when coupled with the MBR, significantly improve the stability and efficiency of this system. We selected environmental bacterial strains which oxidize ammonia and nitrites and produce protease, amylase, and cellulase for the development and testing of a novel biologically enhanced MBR (eMBR). We compared the eMBR with the activated sludge MBR. With the eMBR, the average values of effluent quality were: chemical oxygen demand (COD), 40 mg/l(average efficiency of removal 90.0%); and NH4 +–N, 0.66 mg/l(average efficiency of removal 99.4%). Effluent qualities met the standard and were stable during the entire 90 days of this study. For the activated sludge MBR, the COD removal rate was 91.7%, and the NH4 +–N removal (94.8%) was less than that of the eMBR. Start-up time for the eMBR was only 24–48 h, much shorter than the 7–8 days required to initiate function of the standard MBR. The biomass concentrations of total heterotrophic bacteria and autotrophic bacteria in the eMBR did not fluctuate significantly during the course of the study. Various kinds of microorganisms will establish an ecological balance in the reactor. Compared with the activated sludge MBR, the eMBR not only produced an excellent and stable quality of effluent but also resulted in a shorter time to start-up and significantly improved the efficiency of NH4 +–N removal.







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Bakir HA (2001) Sustainable wastewater management for small communities in the Middle East and North Africa. J Environ Manag 61:319–328
Chen SM, Zheng FS (1985) Experimentation of aquatic microbiology. Ocean, Beijing, China
Chiemchaisri C, Wong YK, Urase T, Yamamoto K (1992) Organic stabilization and nitrogen removal in membarne separation bioreactor for domestic wastewater treatment. Water Sci Technol 25:231–240
Cicek N (2003) A review of membrane bioreactors and their potential application in the treatment of agricultural wastewater. Can Biosyst Eng 45:637–649
Cicek N, Franco JP, Suidan MT, Urbain V (1998) Using a membrane bioreactor to reclaim wastewater. J AWWA 90:105–113
Clesceri LS, Greenberg AE, Eaton AD (eds) (1998) Standard methods for the examination of water and wastewater, 20th edn. APHA, Washington, DC
DiGiano FA, Andreottola G, Adham S, Buckley C, Cornel P, Daigger GT, Fane AG, Galil N, Jacangelo J, Pollice A, Rittmann BE, Rozzi A, Stephenson T, Ojang Z (2004) Membrane bioreactor technology. http://www.scienceinafrica.co.za/2004/june/membrane.htm
General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China (2002) Water quality standard for urban miscellaneous water consumption (GB/T 18920-2002). Beijing
Giraud E, Brauman A, Keleke S, Lelong B, Raimbault M (1991) Isolation and physiological study of an amylolytic strain of Lactobacillus plantarum. Appl Microbiol Biotechnol 36:379–383
Hankin L, Anagnostakis SL (1977) Solid media containing carboxymethylcellulose to detect Cx cellulase activity of microorganisms. J Gen Microbiol 98:109–115
Higa T (1991) Effective microorganisms: a biotechnology for mankind. In: Parr JF, Hornick SB, Whitman CE (eds) Proceedings of the first international conference on Kyusei nature farming. US Department of Agriculture, Washington, DC, USA, pp 8–14
Higa T, Wididana GN (1991) The concept and theories of effective microorganisms. In: Parr JF, Hornick SB, Whitman CE (eds) Proceedings of the first international conference on Kyusei nature farming. US Department of Agriculture, Washington, DC, USA, pp 118–124
Li XY, Chu HP (2003) Membrane bioreactor for the drinking water treatment of polluted surface water supplies. Water Res 37:4781–4791
Liu R, Huan X, Qian Y, Zhang MM (1999) The pilot study on treatment of domestic wastewater using by MBR. Water Wastewater 25:1–4
Muller EB, Stouthamer AH, Verseveld HW, Eikelboom DH (1995) Aerobic domestic waste water treatment in a pilot plant with complete sludge retention by cross-flow filtration. Water Res 29:1179–1189
Olson GE (2004) Hunters Point Shipyard Decentralized Wastewater Treatment Study. Project no. WU-HT-01-34. Prepared for the National Decentralized Water Resources Capacity Development Project, Washington University, St. Louis, MO, by the San Francisco Public Utilities Commission, San Francisco, CA
Rosenberger S, Kruger U, Witzig R, Manz W, Szewzyk U, Kraume M (2002) Performance of a bioreactor with submerged membrane for aerobic treatment of municipal waste water. Water Res 36:413–420
State Environmental Protection Administration of China (2004) Report on the environmental conditions in China
Stephenson T, Judd S, Jefferson B, Brindle K (2000) Membrane bioreactors for wastewater treatment. IWA, London, pp 59–61
Sundaravadivel M, Vigneswaran S (2001) Wastewater collection and treatment technologies for semi-urban areas of India: a case study. Water Sci Technol 43:329–336
Teather RM, Wood PJ (1982) Use of Congo red–polysaccharide interactions in enumeration and characterization of cellulolytic bacteria from bovine rumen. Appl Environ Microbiol 43:777–780
Ueda T, Hata K (1999) Domestic wastewater treatment by a submerged membrane bioreactor with gravitational filtration. Water Res 33:2888–2892
Van de Roest HF, Lawrence DP, Van Bentem AGN (2002) Membrane bioreactors for municipal wastewater treatment. STOWA Report. IWA, London
Visvanathan C, Aim RB, Parameshwaran K (2000) Membrane separation bioreactor for wastewater treatment. Crit Rev Environ Sci Technol 30:1–48
Williams CM, Richter CS, MacKenzie JM, Shih JCH (1990) Isolation, identification and characterisation of a feather degrading bacterium. Appl Environ Microbiol 56:1509–1515
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This work presents a part of the results of the Nation High Technology Research and Development Program of China (“863” Projects, 2002AA601240) and the key Project of Sci&Tech of Tianjin (No. 023180711).
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Jin, M., Wang, XW., Gong, TS. et al. A novel membrane bioreactor enhanced by effective microorganisms for the treatment of domestic wastewater. Appl Microbiol Biotechnol 69, 229–235 (2005). https://doi.org/10.1007/s00253-005-0108-5
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DOI: https://doi.org/10.1007/s00253-005-0108-5


