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The arginine deiminase locus of Oenococcus oeni includes a putative arginyl-tRNA synthetase ArgS2 at its 3′-end

  • Applied Microbial and Cell Physiology
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Abstract

Oenococcus oeni is the most important lactic acid bacteria of the winemaking process involved in malolactic fermentation. Most O. oeni strains are able to catabolyze arginine via the arginine deiminase (ADI) pathway. The arcR, A, B, C, D1, and D2 cluster of O. oeni bacteria has been characterized. Here, we completed the ADI locus sequence. Downstream of arcD2 gene, we found an additional gene which encodes a putative arginyl-tRNA synthetase (argS2). It is not the same arginyl-tRNA synthetase which was sequenced in O. oeni MCW strain. Transcriptional analyses have shown that argS2 was induced by arginine. In addition, systematic polymerase chain reaction amplification of each arc gene and argS2 has provided a characteristic feature of the ADI locus within the O. oeni species: all genes of ADI locus are present or absent according to the strains.

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Acknowledgements

The authors are grateful to Dr. Caroline Duchaine for useful discussions. This work was supported by a grant from the Ministère de l'Education Nationale, de la Recherche et de la Technologie. M. Angelica Ganga was the recipient of a Connicyt-French Embassy (Chile) fellowship.

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  1. Benjamin Nehmé

    Present address: Institut Universitaire de Cardiologie et de Pneumologie, Hôpital Laval, Université Laval, 2725 Chemin Ste-Foy, Ste-Foy, Quebec, G1V 4G5, Canada

Authors and Affiliations

  1. Faculté d'Oenologie, UMR INRA 1219 oenologie-ampélologie, Université de Bordeaux II, 351 cours de la Libération, 33405, Talence Cedex, France

    Benjamin Nehmé & Aline Lonvaud-Funel

  2. Departamento de Gestión Agraria, Facultad Tecnológica, Centro de Estudios en Ciencia y Tecnología de los Alimentos, Universidad de Santiago de Chile, Santiago, Chile

    Maria Angélica Ganga

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  1. Benjamin Nehmé
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  2. Maria Angélica Ganga
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  3. Aline Lonvaud-Funel
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Correspondence to Benjamin Nehmé.

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Nehmé, B., Ganga, M.A. & Lonvaud-Funel, A. The arginine deiminase locus of Oenococcus oeni includes a putative arginyl-tRNA synthetase ArgS2 at its 3′-end. Appl Microbiol Biotechnol 70, 590–597 (2006). https://doi.org/10.1007/s00253-005-0095-6

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  • DOI: https://doi.org/10.1007/s00253-005-0095-6

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