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Applied Microbiology and Biotechnology

, Volume 68, Issue 2, pp 183–188 | Cite as

Induction and potentiation of diterpenoid tanshinone accumulation in Salvia miltiorrhiza hairy roots by β-aminobutyric acid

  • Xiuchun Ge
  • Jianyong WuEmail author
Biotechnological Products and Process Engineering

Abstract

The non-protein amino acid β-aminobutyric acid (BABA) is a proven inducer of plant defense against pathogens. This work examines its effect on the production of diterpenoid tanshinones in Salvia miltiorrhiza hairy root cultures, both separately and in combination with a yeast elicitor (YE, the carbohydrate fraction of yeast extract). In the absence of YE, BABA at 0.1, 1 and 2 mM caused a dose-dependent enhancement of tanshinone accumulation, with up to a 4.5-fold increase (from 0.24 to 1.09 mg/g DW) in total content of three major tanshinones (cryptotanshinone, tanshinone I and tanshinone IIA) in the hairy roots. The combination of BABA with YE treatment further enhanced tanshinone production, but only when the BABA treatment was applied to the culture a few days before the YE treatment. Compared with methyl jasmonate, BABA was more effective in enhancing tanshinone production. A 3-day pretreatment with 1 mM BABA followed by YE-treatment, increased the total tanshinone content of roots by 9.4 times to 2.26 mg/g cells, and the volumetric tanshinone yield of culture by 6.3 times (from 3.2 to 20.1 mg/l). The results suggest that BABA can strongly potentiate elicitor-induced secondary metabolism in plant tissue cultures.

Keywords

Hairy Root Hairy Root Culture Secondary Metabolite Production Tanshinone Cryptotanshinone 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgements

This work was supported by grants from the Hong Kong Polytechnic University (PD93 and ASD fund) and the Areas of Excellence Scheme established under the University Grants Committee of the Hong Kong SAR, China (AoE/P-10/2001)

References

  1. Chen H, Chen F (1999) Effects of methyl jasmonate and salicylic acid on cell growth and cryptotanshinone formation in TI-transformed Salvia miltiorrhiza cell suspension cultures. Biotechnol Lett 21:803–807Google Scholar
  2. Chen H, Chen F, Zhang YL, Song JY (1999) Production of lithospermic acid B and rosmarinic acid in hairy root cultures of Salvia miltiorrhiza. J Ind Microbiol Biotechnol 22:133–138Google Scholar
  3. Chen H, Chen F, Chiu CK, Lo MY (2001) The effect of yeast elicitor on the growth and secondary metabolism of hairy root cultures of Salvia miltiorrhiza. Enzyme Microb Technol 22:133–138Google Scholar
  4. Conrath U, Pieterse CMJ, Mauch-Mani B (2002) Priming in plant–pathogen interactions. Trends Plant Sci 7:210–216CrossRefGoogle Scholar
  5. Creelman RA, Mullet JE (1997) Biosynthesis and action of jasmonates in plants. Annu Rev Plant Physiol Plant Mol Biol 48:355–381CrossRefPubMedGoogle Scholar
  6. Doran PM (1997) Hairy roots: culture and applications. Harwood, AmsterdamGoogle Scholar
  7. Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F (1956) Colorimetric method for determination of sugar and related substances. Anal Chem 68:350–356Google Scholar
  8. Eilert U (1987) Elicitation: methodology and aspects of application. In: Constabel F, Vasil I (eds) Cell culture and somatic cell genetics of plants, vol 4. Academic Press, San Diego, Calif., pp 153–196Google Scholar
  9. Enyedi AJ, Yalpani N, Silverman P, Raskin I (1992) Signal molecules in systemic plant resistance to pathogens and pests. Cell 70:879–886CrossRefPubMedGoogle Scholar
  10. Gundlach H, Mueller MJ, Kutchan TM, Zenk MH (1992) Jasmonic acid is a signal transducer in elicitor-induced plant cell cultures. Proc Natl Acad Sci USA 89:2389–2393PubMedGoogle Scholar
  11. Hahn MG, Albersheim P (1978) Host–pathogen interactions. XIV. Isolation and partial characterization of elicitor from yeast extract. Plant Physiol 62:107–111Google Scholar
  12. Hu ZB, Alfermann AW (1993) Diterpenoid production in hairy root cultures of Salvia miltiorrhiza. Phytochemistry 2:699–703Google Scholar
  13. Jakab G, Cottier V, Toquin V, Rigoli G, Zimmerli L, Métraux JP, Mauch-Mani B (2001) β-Aminobutyric acid-induced resistance in plants. Eur J Plant Pathol 107:29–37Google Scholar
  14. Katz VA, Thulke OU, Conrath U (1998) A benzothiadiazole primes parsley cells for augmented elicitation of defense responses. Plant Physiol 117:1333–1339Google Scholar
  15. Kauss H, Jeblick W (1995) Pretreatment of parsley suspension cultures with salicylic acid enhances spontaneous and elicited production of H2O2. Plant Physiol 108:1171–1178Google Scholar
  16. Kauss H, Krause K, Jeblick W (1992) Methyl jasmonate conditions parsley suspension cells for increased elicitation of phenylpropanoid defense responses. Biochem Biophys Res Commun 189:304–308PubMedGoogle Scholar
  17. Linden JC, Phisalaphong M (2000) Oligosaccharides potentiate methyl jasmonate-induced production of paclitaxel in Taxus canadensis. Plant Sci 158:41–51Google Scholar
  18. Tang W, Eisenbrand G (1992) Chinese drugs of plant origin: chemistry, pharmacology, and use in traditional and modern medicine. Springer, Berlin Heidelberg New York, pp 891–902Google Scholar
  19. Zhang C, Qiong Y, Cheuk W, Wu J (2004) Enhancement of tanshinone production in Salvia miltiorrhiza hairy root culture by Ag+ and nutrient feeding. Planta Medica 70:147–151Google Scholar
  20. Zimmerli L, Jakab G, Métraux JP, Mauch-Mani B (2000) Potentiation of pathogen-specific defense mechanisms in Arabidopsis by β-aminobutyric acid. Proc Natl Acad Sci USA 97:12920–12925Google Scholar
  21. Zimmerli L, Metraux JP, Mauch-Mani B (2001) β-Aminobutyric acid-induced protection of Arabidopsis against the necrotrophic fungus Botrytis cinerea. Plant Physiol 126:517–523Google Scholar

Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  1. 1.Department of Applied Biology and Chemical TechnologyThe Hong Kong Polytechnic UniversityHung Hom, KowloonHong Kong
  2. 2.Department of Plant Protection, College of Agriculture and BiotechnologyZhejiang UniversityHangzhouChina

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