Applied Microbiology and Biotechnology

, Volume 68, Issue 2, pp 259–265 | Cite as

Exopolysaccharide biosynthesis by Lactobacillus helveticus ATCC 15807

  • M. I. Torino
  • F. Mozzi
  • G. Font de ValdezEmail author
Applied Microbial and Cell Physiology


Exopolysaccharide (EPS) production and the activities of the enzymes involved in sugar nucleotide biosynthesis in Lactobacillus helveticus ATCC 15807 under controlled pH conditions were investigated. Batch fermentations using lactose as energy source showed higher EPS synthesis by L. helveticus ATCC 15807 at pH 4.5 with respect to pH 6.2, the enzyme α-phosphoglucomutase (α-PGM) being correlated with both total and specific EPS production. When glucose was used as carbon source instead of lactose, the lower EPS synthesis obtained was linked to a decrease in α-PGM and galactose 1-phosphate-uridyltransferase (GalT) activities, the reduction of the latter being more pronounced. Higher EPS production by L. helveticus ATCC 15807 at the acidic constant pH of 4.5 requires that both α-PGM and GalT activities are high. These enzymes are needed to synthesize UDP-glucose and UDP-galactose for supplying the corresponding monomers for EPS biosynthesis. Although differences are observed in EPS production by this strain regarding the energy source (lactose or glucose), the monomeric composition of the polymers produced is independent of the carbohydrate used. The obtained results contribute to a better understanding of the physiological factors that affect EPS biosynthesis by lactobacilli, which could help in the correct handling of the fermentation parameters within the fermented dairy industry.


Lactic Acid Bacterium Fermented Milk Sugar Nucleotide Mozzarella Cheese GalT Activity 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



The authors acknowledge the financial support of the Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), the Agencia Nacional de Promoción Científica y Tecnológica (ANPCyT) and CIUNT in Argentina. The authors are very grateful to Prof. Dr. ir. Luc de Vuyst and Dr. ir. Frederik Vaningelgem (IMDO, VUB, Brussels, Belgium) for their help in the EPS monomeric composition analysis


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Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  1. 1.Centro de Referencia para Lactobacilos (CERELA-CONICET)San Miguel de TucumánArgentina
  2. 2.Cátedra de Microbiología SuperiorUniversidad Nacional de TucumánSan Miguel de TucumánArgentina

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