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Applied Microbiology and Biotechnology

, Volume 68, Issue 2, pp 242–250 | Cite as

SspA, an outer membrane protein, is highly induced under salt-stressed conditions and is essential for growth under salt-stressed aerobic conditions in Rhodobacter sphaeroides f. sp. denitrificans

  • M. TsuzukiEmail author
  • XY. Xu
  • K. Sato
  • M. Abo
  • M. Arioka
  • H. Nakajima
  • K. Kitamoto
  • A. Okubo
Applied Microbial and Cell Physiology

Abstract

We have previously shown that an outer membrane protein, SspA, is prominently induced by salt stress in a photosynthetic bacterium, Rhodobacter sphaeroides f. sp. denitrificans IL106 (R. sphaeroides). In this study, we investigated the physiological role of SspA under various stress conditions. Using recombinant SspA expressed in Escherichia coli as an antigen, the polyclonal antiserum of SspA was prepared. Western blot analysis demonstrated that SspA was highly induced by salt stress under both anaerobic and aerobic conditions. SspA was also induced, but to a lesser extent, by osmotic and acid stress. It is reduced under heat and cold compared to non-stressed conditions. While sspA-disrupted R. sphaeroides grew normally under anaerobic conditions in either the presence or absence of stress, it displayed significantly retarded growth under aerobic conditions in the dark, especially when osmotic or salt stress were imposed. In addition, the sspA disruptant, but not the wild type, formed cell aggregates when grown under both anaerobic and aerobic conditions, and this phenotype was significantly enhanced under salt-stressed aerobic conditions. Together, our findings suggest that SspA is critical under salt-stressed, aerobic growth conditions.

Keywords

Salt Stress Aerobic Condition Synechocystis Glycine Betaine Ectoine 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgements

We are grateful to Drs. K. Matsuura, K. Shimada, K. V. P. Nagashima, (Tokyo Metropolitan University) and Dr. S. Masuda (RIKEN) for the gift of the E. coli strains, plasmids, and for expert technical assistance

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Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • M. Tsuzuki
    • 1
    Email author
  • XY. Xu
    • 1
  • K. Sato
    • 1
  • M. Abo
    • 1
  • M. Arioka
    • 2
  • H. Nakajima
    • 2
  • K. Kitamoto
    • 2
  • A. Okubo
    • 1
  1. 1.Department of Applied Biological Chemistry, Graduate School of Agricultural and Life SciencesThe University of TokyoBunkyo-kuJapan
  2. 2.Department of Biotechnology, Graduate School of Agricultural and Life SciencesThe University of TokyoBunkyo-kuJapan

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