Abstract
An efficient fedbatch process for the production of Lactobacillus kefir DSM 20587 cells was developed. An improvement in space time yield of 270% (3.7 gDCW l−1 day−1) and in final enzyme activity of 440% (9.1 U/ml) was achieved on a 150 l scale by controlling the oxygen transfer rate to 7–9 mmol l−1 h−1. The cells exhibited good and highly stereoselective reducing activities against tert-butyl 6-chloro-3,5-dioxohexanoate. tert-Butyl (3R,5S)-6-chloro-dihydroxyhexanoate, a chiral building block for HMG-CoA reductase inhibitor synthesis, was produced with 47.5% yield and >99% ee at C33 and C55 in a simple batch biotransformation process.
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Acknowledgements
The authors are very grateful to M. Mueller for discussion and for providing us with the dioxohexanoate and hydroxyhexanoate products. We would like to thank W. Hummel and T. Daussmann (Juelich Fine Chemicals, Germany) for helpful discussions. This work was supported by the Deutsche Bundesstiftung Umwelt (grant no. AZ 13040/03)
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Pfruender, H., Amidjojo, M., Hang, F. et al. Production of Lactobacillus kefir cells for asymmetric synthesis of a 3,5-dihydroxycarboxylate. Appl Microbiol Biotechnol 67, 619–622 (2005). https://doi.org/10.1007/s00253-004-1837-6
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DOI: https://doi.org/10.1007/s00253-004-1837-6