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Lactulose production by β-galactosidase in permeabilized cells of Kluyveromyces lactis

Abstract

Lactulose production from lactose and fructose was investigated with several commercial β-galactosidases. The enzyme from Kluyveromyces lactis exhibited the highest lactulose productivity among the β-galactosidases tested. The reaction conditions for lactulose production were optimized using cells that had been permeabilized by treatment with 50% (v/v) ethanol: cell concentration, 10.4 g l−1; concentration of substrates, 40% (w/v) lactose and 20% (w/v) fructose; temperature, 60°C; pH 7.0. Under these conditions, the permeabilized cells produced approximately 20 g l−1 lactulose in 3 h with a lactulose productivity of 6.8 g l−1 h−1. These results represent 1.3- and 2.1-fold increases in lactulose concentration and productivity compared with untreated washed cells. This is the first reported trial of enzymatic synthesis of lactulose using permeabilized yeast cells.

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Acknowledgements

This study was supported by a grant of the 21C Frontier Project for Microbial Genomics, Ministry of Science and Technology, Republic of Korea.

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Correspondence to D.-K. Oh.

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Lee, YJ., Kim, C.S. & Oh, DK. Lactulose production by β-galactosidase in permeabilized cells of Kluyveromyces lactis . Appl Microbiol Biotechnol 64, 787–793 (2004). https://doi.org/10.1007/s00253-003-1506-1

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Keywords

  • Fructose
  • Lactose
  • Lactis
  • Permeabilized Cell
  • Lactulose