Abstract
Pleurotus pulmonarius fruit body primordia (FBP) formed on potato extract agar (PEA) and wheat straw-based media (WS) were studied. When grown on PEA, FBP hyphae had a large number of vacuoles and less stainable cytoplasmic material. In contrast, when grown on WS, there was abundant cytoplasmic material and fewer vacuoles. FBP grown on WS had a more well-defined mushroom shape than those grown on PEA. The average hyphal diameters on PEA and WS were 4.15 and 3.52 µm, respectively; the average hyphal lengths were 11.75 and 11.80 µm, respectively. The control mechanisms regulating formation of the normal shape and size of FBP might be more dependent on the amount of cytoplasmic material than on the physical volume of the hyphal compartment. Since the ultrastructure of the hyphal compartment, which makes up the substance of the FBP, depends on the substrate in which the FBP has been grown, the composition of the substrate may be important for manipulating the nutritive and organoleptic characteristics of the mushroom crop.
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References
Booth C (1971) The genus Fusarium. Commonwealth Mycological Society. Kew, England
Kües U, Liu Y (2000) Fruiting body production in basidiomycetes. Appl Microbiol Biotechnol 54:141–152
Macaya-Lizano AV (1975) Pleurotus ostreatus (Jacq. ex Fr.) quélet, formes et especes affines. Comportement cultural et sistématique. Rev Mycol 39:1–57
McMeekin D (1991) Basidiocarp formation in Asterophora lycoperdoides. Mycologia 83:220–223
Moore D (1998) Tolerance of imprecision in fungal morphogenesis. In: Van Griensven L JLD, Visser J (eds) Proceedings of the Fourth Conference on the Genetics and Cellular Biology of Basidiomycetes. The Mushroom Experimental Station, Horst The Netherlands, pp 3–19
Niederpruem DJ, Hobbs H, Henry L (1964) Nutritional studies of development in Schizophyllum commune. J Bacteriol 88:1721–1728
Sánchez C (1998) Morphogenesis of mushroom fungi: ultrastructural, physiological and histological study of Pleurotus species. PhD Dissertation The University of Manchester, Manchester UK
Sánchez C, Moore D (1999) Conventional histological stains selectively stain fruit body initials for both light and scanning electron microscopy. Mycol Res 103:315-318
Wessels JGH (1993) Fruiting in higher fungi. Adv Microb Physiol 34:147–202
Zadrazil F (1976) The ecology and industrial production of Pleurotus ostreatus, Pleurotus florida, Pleurotus cornucopiae, and Pleurotus eryngii. Mushroom Sci 9:621–652
Acknowledgements
The author wishes to thank the Mexican National Council of Science and Technology (CONACYT) and The British Council for supporting this research, and Dr. S.W. Chiu for kindly providing P. pulmonarius PL27. The electron microscopy unit at the University of Manchester is also thanked for use of their equipment. The experiments were undertaken under the current laws of the Mexican Republic.
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Sánchez, C. Influence of the substrate on the ultrastructure of Pleurotus pulmonarius fruit body primordia. Appl Microbiol Biotechnol 64, 691–694 (2004). https://doi.org/10.1007/s00253-003-1496-z
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DOI: https://doi.org/10.1007/s00253-003-1496-z