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Novel bioreduction system for the production of chiral alcohols

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Abstract.

Chiral alcohols are useful intermediates for many pharmaceuticals and chemicals. Enzymatic asymmetric reduction of prochiral carbonyl compounds is a promising method for producing chiral alcohols. There have been many attempts to construct bioreduction systems for the industrial production of chiral alcohols. This review focuses on the establishment of a novel bioreduction system using an Escherichia coli transformant co-expressing genes for carbonyl reductase and cofactor-regeneration enzyme. This bioreduction system could be useful as an all-purpose catalyst for asymmetric reduction reactions.

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Acknowledgements.

This work was supported in part by a Grant-in-Aid for Scientific Research (number 13853009, to S.S. and number 14656039, to M.K.) from the Japan Society for the Promotion of Science. This work was carried out as part of the Project for Development of a Technological Infrastructure for Industrial Bioprocesses on R&D of New Industrial Science and Technology Frontiers, Ministry of Economy, Trade and Industry (METI), entrusted by the New Energy and Industrial Technology Development Organization (NEDO) to S.S.

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Authors and Affiliations

  1. Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Sakyo-ku, 606-8502, Kyoto, Japan

    M. Kataoka & S. Shimizu

  2. Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Uji, 611-0011, Kyoto, Japan

    K. Kita

  3. Department of Bioscience, Fukui Prefectural University, 4-1-1 Kenjyojima, Matsuoka-cho, 910-1195, Fukui, Japan

    M. Wada

  4. Fine Chemical Research Laboratories, Kaneka Corporation, 1-8 Miyamae, Takasago, Hyogo 676-8688, Japan

    Y. Yasohara & J. Hasegawa

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  1. M. Kataoka
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  2. K. Kita
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  4. Y. Yasohara
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  5. J. Hasegawa
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Correspondence to S. Shimizu.

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Kataoka, M., Kita, K., Wada, M. et al. Novel bioreduction system for the production of chiral alcohols. Appl Microbiol Biotechnol 62, 437–445 (2003). https://doi.org/10.1007/s00253-003-1347-y

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  • DOI: https://doi.org/10.1007/s00253-003-1347-y

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