Abstract
Optimisation of membrane properties of alginate microcapsules is a key factor for the application of microencapsulation techniques to bioartificial organ elaboration. Coacervation and layer-by-layer processes involving additional biopolymers have been extensively studied. Recently, the use of silica as a membrane-forming agent was investigated. This approach was rendered possible by the development of biocompatible routes to silica formation. The composites exhibit enhanced mechanical and thermal stability as well as suitable diffusion properties. Moreover, encapsulated enzymes and cells retain their biological activities. Similarly, silica can be associated to many other biopolymers, opening a promising route for new biocomposites design and biotechnology applications.
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Armanini L, Carturan G, Boninsegna S, Dal Monte R, Muraca M (1999) SiO2 entrapment of animal cells. Part 2: protein diffusion through collagen membranes coated with sol-gel SiO2. J Mater Chem 9:3057–3060
Avnir D, Braun S, Lev O, Ottolenghi M (1994) Enzymes and other proteins entrapped in sol-gel materials. Chem Mater 6:1605–1614
Barbotin JN, Nava Saucedo JE (1996) Bioencapsulation of living cells by entrapment in polysaccharide gels. In: Dimitriu S (ed) Polysaccharides: structural diversity and functional versatility. Marcel Dekker, New York, pp 749–774
Bartkowiak A, Hunkeler D (1999) Alginate-oligochitosan microcapsules: a mechanistic study relating membrane and capsule properties to reaction conditions. Chem Mater 11:2486–2492
Bhatia RB, Brinker CJ, Gupta AK, Singh AK (2000) Aqueous sol-gel process for protein encapsulation. Chem Mater 12:2434–2441
Boninsegna S, Dal Toso R, Dal Monte R, Carturan G (2003) Alginate microspheres loaded with animal cells and coated by a siliceous layer. J Sol-Gel Sci Technol 26:1154–1157
Brasack I, Bottcher H, Hempel U (2000) Biocompatibility of modified silica-protein composite layers. J Sol-Gel Sci Technol 19:479–482
Brinker CJ, Scherrer G (1990) The physics and chemistry of sol-gel processing. Academic, Boston
Carturan G, Dal Monte R, Muraca M (2000) SiO2 entrapment of animal cells for hybrid bioartificial organs. Mat Res Soc Symp Proc 628:CC10.1.1–14
Chandra R, Rutsgi R (1998) Biodegradable polymers. Prog Polym Sci 23:1273–1335
Coradin T, Mercey E, Lisnard L, Livage J (2001) Design of silica-coated microcapsules for bioencapsulation. Chem Commun 2496–2497
Coradin T, Coupé A, Livage J (2002) Influence of DNA, alginate, lysozyme and bovine serum albumin on sodium silicate condensation. Mat Res Soc Symp Proc 724:N7.20.1–6
Coradin T, Livage J (2003) Synthesis and characterization of alginate/silica biocomposites. J Sol-Gel Sci Technol 26:1165–1168
Decher G (1997) Fuzzy nanoassemblies: toward layered polymeric multicomposites. Science 227:1232–1237
Ellerby LM, Nishida CR, Nishida F, Yamanaka SA, Dunn B, Valentine JS, Zink JI (1992) Encapsulation of proteins in transparent porous silicate glasses prepared by the sol-gel method. Science 255:1113–1115
Ferrer ML, del Monte F, Levy D (2002) A novel and simple alcohol-free sol-gel route for encapsulation of labile proteins. Chem Mater 14:3619–3621
Fukushima Y, Okamura K, Imai K, Motai H (1988) A new immobilization technique of whole cells and enzymes with colloidal silica and alginate. Biotechnol Bioeng 32:584–594
Gill I, Ballesteros A (1998) Encapsulation of biologicals within silicate, siloxane and hybrid sol-gel polymers: an efficient and generic approach. J Am Chem Soc 120:8587–8598
Gill I, Ballesteros A (2000) Bioencapsulation within synthetic polymers (Part 1): sol-gel encapsulated biologicals . Trends Biotechnol 18:282–296
Goosen MFA, O'Shea GM, Gharapetian HM, Chou S, Sun AM (1985) Optimization of microcapsulation parameters: semipermeable microcapsules as a bioartificial pancreas. Biotechnol Bioeng 27:146–150
Heichal-Segal O, Rappoport S, Braun S (1995) Immobilization in alginate-silicate sol-gel matrix protects β-galactosidase against thermal and chemical denaturation. Bio/technology 13:798–800
Hertzberg S, Moen E, Vogelsang C, Ostgaard K (1995) Mixed photo-cross-linked polyvinyl alcohol and calcium alginate gels for cell entrapment. Appl Microbiol Biotechnol 43:10–17
Iler RK (1979) The chemistry of silica: solubility, polymerisation, colloid and surface properties, biochemistry. Wiley-Interscience, New York
Judenstein P, Sanchez C (1996) Hybrid organic-inorganic materials: a land of multidisciplinarity. J Mater Chem 6:511–525
Lim F, Sun A (1980) Microencapsulated islets as bioartificial endocrine pancreas. Science 210:908–910
Livage J. Coradin T, Roux C (2001) Encapsulation of biomolecules in silica gels. J Phys Condens Matter 13:R673–691
Lloyd-George I, Chang TMS (1995) Characterization of free and alginate-polylysine-alginate microencapsulated Erwinia herbicola for the conversion of ammonia, pyruvate, and phenol into L-Tyrosine. Biotechnol Bioeng 48:706–714
Nassif N, Rager MN, Bouvet O, Roux C, Coradin T, Livage J (2002) Living bacteria in silica gels. Nature Materials 1:42–45
Nava Saucedo JE, Audras B, Jan S, Bazinet CE, Barbotin JN (1994) Factors affecting densities, distribution and growth patterns of cells inside immobilization supports. FEMS Microbiol Lett 14:93–98
Poncelet D, Dulieu C, Jacquot M (2000) Description of the immobilisation procedures. In: Wijffels R (ed) Immobilized cells. Springer Lab Manual, Heidelberg, pp15–30
Reetz M (1997) Entrapment of biocatalysts in hydrophobic sol-gel materials for use in organic chemistry. Adv Mater 9:943–954
Ren L, Tsuru K, Hayakawa S, Osaka A (2002) Novel approach to fabricate porous gelatin-siloxane hybrids for bone tissue engineering. Biomaterials 23:4765–4773
Sabra V, Zeng AP, Deckwer WD (2001) Bacterial alginate: physiology, product quality and process aspects. Appl Microbiol Biotechnol 56:315–325
Sakai S, Ono, T, Ijima H, Kawakami K (2001) Synthesis and transport characterization of alginate/aminopropyl-silicate/aginate microcapsule: application to bioartificial pancreas. Biomaterials 22:2827–2834
Sakai S, Ono, T, Ijima H, Kawakami K (2002a) In vitro and in vivo evaluation of alginate/sol-gel synthesized aminopropyl-silicate/alginate membrane for bioartificial pancreas. Biomaterials 23:4177–4183
Sakai S, Ono, T, Ijima H, Kawakami K (2002b) Alginate/aminopropyl-silicate/aginate membrane immunoisolatability and insulin secretion of encapsulated islets. Biotechnol Prog 18:401–403
Sakai S, Ono T, Ijima H, Kawakami K (2002c) Aminopropyl-silicate membrane for microcapsule-shaped bioartificial organs: control of molecular permeability. J Memb Sci 202:73–80
Schneider S, Feilen PJ, Slotty V, Kampfner D, Preuss S, Berger S, Beyer J, Pommersheim R (2001) Multilayer capsules: a promising microencapsulation system for transplantation of pancreatic islets. Biomaterials 22:1961–1970
Sglavo VM, Carturan G, Dal Monte R, Muraca M (1999) SiO2 entrapment of animal cells. Part 1: mechanical features of sol-gel SiO2 coatings. J Mater Sci 34:3587–3590
Smidsrod O, Skjak-Braek G (1990) Alginate as immobilization matrix for cells. Trends Biotechnol 8:71–78
Tamura H, Tsuruta Y, Tokura S (2002) Preparation of chitosan-coated alginate filament. Mater Sci Eng C 20:143–147
Uludag H, De Vos P, Tresco PA (2000) Technology of mammalian cell encapsulation. Adv Drug Deliv Rev 42:29–64
Wang YJ (2000) Development of new polycations for cell encapsulation with alginate. Mater Sci Eng C 13:59–63
Willaert RG, Baron GV (1996) Gel entrapment and microencapsulation: methods, applications and engineering principles. Rev Chem Eng 12:1-205
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Coradin, T., Nassif, N. & Livage, J. Silica–alginate composites for microencapsulation. Appl Microbiol Biotechnol 61, 429–434 (2003). https://doi.org/10.1007/s00253-003-1308-5
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DOI: https://doi.org/10.1007/s00253-003-1308-5