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Corn fiber hydrolysis by Thermobifida fusca extracellular enzymes

Applied Microbiology and Biotechnology volume 61, pages 352–358 (2003)Cite this article

Abstract.

Thermobifida fusca was grown on cellulose (Solka-Floc), xylan or corn fiber and the supernatant extracellular enzymes were concentrated. SDS gels showed markedly different protein patterns for the three different carbon sources. Activity assays on a variety of synthetic and natural substrates showed major differences in the concentrated extracellular enzyme activities. These crude enzyme preparations were used to hydrolyze corn fiber, a low-value biomass byproduct of the wet milling of corn. Approximately 180 mg of reducing sugar were produced per gram of untreated corn fiber. When corn fiber was pretreated with alkaline hydrogen peroxide, up to 429 mg of reducing sugars were released per gram of corn fiber. Saccharification was enhanced by the addition of β-glucosidase or by the addition of a crude xylanase preparation from Aureobasidium sp.

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Acknowledgements.

This work was conducted under Specific Cooperative Agreement 58-3620-9-117 between Cornell University and the Agricultural Research Service of the United States Department of Agriculture (USDA). The authors are grateful for the expert technical contributions of Melinda S. Nunnally and Mark Cheng. Product names are necessary to report factually on available data. However, the USDA neither guarantees nor warrants the standard of the product; and the use of the name by USDA implies no approval of the product to the exclusion of others that may also be suitable.

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Affiliations

  1. Department of Molecular Biology and Genetics, Cornell University, 458 Biotechnology Building, Ithica, NY 14850, USA

    D. Irwin & D. B. Wilson

  2. Fermentation Biotechnology Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, United States Department of Agriculture, Peoria, IL 61604, USA

    T. D. Leathers

  3. Office of International Programs, Agricultural Research Service, United States Department of Agriculture, Beltsville, MD 20705, USA

    R. V. Greene

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  1. D. Irwin
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  2. T. D. Leathers
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  3. R. V. Greene
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  4. D. B. Wilson
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Correspondence to D. B. Wilson.

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Irwin, D., Leathers, T.D., Greene, R.V. et al. Corn fiber hydrolysis by Thermobifida fusca extracellular enzymes. Appl Microbiol Biotechnol 61, 352–358 (2003). https://doi.org/10.1007/s00253-002-1210-6

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  • DOI: https://doi.org/10.1007/s00253-002-1210-6

Keywords

  • Cellulase
  • Cellobiose
  • Fusca
  • Corn Fiber
  • Total Reduce Sugar