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Applied Microbiology and Biotechnology

, Volume 60, Issue 4, pp 489–494 | Cite as

Mono- and dimeric ferulic acid release from brewer's spent grain by fungal feruloyl esterases

  •  C. Faulds
  •  A. Sancho
  •  B. Bartolomé
Original Paper

Abstract.

Ultraflo L, a β-glucanase preparation from Humicola insolens sold for reducing viscosity problems in the brewing industry, exhibited activity against the methyl esters of ferulic, caffeic, p-coumaric and sinapic acids, displaying mainly type-B feruloyl esterase activity. Ultraflo also contained the ability to release 65% of the available ferulic acid (FA) together with three forms of diferulate from brewer's spent grain (BSG). An "esterase-free" Ultraflo preparation greatly enhanced the ability of a feruloyl esterase from Aspergillus niger, AnFAEA, to release FA (from 23 to 47%) and its dimeric forms, especially the 8,5′ benzofuran form, from BSG. While total release of these phenolic acids was not observed, this synergistic enhancement of ferulate release demonstrates that FA and its dimeric forms present in BSG require the addition of more than a xylanase. This suggests either that FA is not solely attached to arabinoxylan in the barley cell wall, or that the cell wall polysaccharides in BSG hinder the accessibility of enzymes to the ferulates, due to processing treatments.

Keywords

Aspergillus Niger Ferulic Acid Esterase Activity Dimeric Form Cell Wall Polysaccharide 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 2002

Authors and Affiliations

  •  C. Faulds
    • 1
  •  A. Sancho
    • 1
  •  B. Bartolomé
    • 2
  1. 1.Food Materials Science Division, Institute of Food Research, Norwich Research Park, Colney, Norwich NR4 7UA, UK
  2. 2.Instituto de Fermentaciones Industriales (CSIC), Juan de Cierva 3, 28006 Madrid, Spain

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