Abstract.
Acinetobacter sp. strain ST-550 was isolated from fumus soil as an efficient indigo producer in the presence of organic solvent. The minimum inhibitory concentration of indole was 0.4 mg/ml for ST-550. ST-550 produced only a small amount of indigo (less than 0.01 µg/ml) when grown in the presence of indole at concentrations of 0.05 to 0.3 mg/ml without any organic solvent. However, ST-550 produced indigo effectively when grown in the presence of a large volume of diphenylmethane and a high level of indole; optimized conditions were 3 ml of a medium containing 0.3 ml diphenylmethane and 2.7 mg indole. Under these conditions, ST-550 produced 0.88 mg indigo (292 µg/ml medium).
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Doukyu, .N., Nakano, .T., Okuyama, .Y. et al. Isolation of an Acinetobacter sp. ST-550 which produces a high level of indigo in a water-organic solvent two-phase system containing high levels of indole. Appl Microbiol Biotechnol 58, 543–546 (2002). https://doi.org/10.1007/s00253-001-0919-y
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DOI: https://doi.org/10.1007/s00253-001-0919-y