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Degradation of biphenyl by Mycobacterium sp. strain PYR-1

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Abstract.

The metabolism of biphenyl by Mycobacterium sp. PYR-1 was investigated. The Mycobacterium sp. degraded >98% of the biphenyl added within 72 h. Analysis of ethyl acetate extracts of the culture medium by HPLC indicated that benzoic acid was the major metabolite. Other products were 4-hydroxybiphenyl, 4-hydroxybenzoic acid, and 5-oxo-5-phenylpentanoic acid. The metabolites were characterized by mass and 1H NMR spectrometry. Identification of benzoic acid and 5-oxo-5-phenylpentanoic acid indicates that biphenyl degradation by Mycobacterium sp. PYR-1 is generally similar to known pathways. A novel alternative metabolic pathway consisted of monooxygenation at C-4 of biphenyl to give 4-hydroxybiphenyl, with subsequent degradation via ring cleavage to 4-hydroxybenzoic acid.

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Authors and Affiliations

  1. Division of Microbiology, National Center for Toxicological Research, U.S. Food and Drug Administration, Jefferson, AR 72079 USA, , , , ,

    J. Moody &  C. Cerniglia

  2. Division of Biochemical Toxicology, National Center for Toxicological Research, U.S. Food and Drug Administration, Jefferson, AR 72079 USA, , , , ,

    D. Doerge

  3. Division of Chemistry, National Center for Toxicological Research, U.S. Food and Drug Administration, Jefferson, AR 72079 USA, , , , ,

    J. Freeman

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  1. J. Moody
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  2. D. Doerge
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  3. J. Freeman
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  4. C. Cerniglia
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Moody, .J., Doerge, .D., Freeman, .J. et al. Degradation of biphenyl by Mycobacterium sp. strain PYR-1. Appl Microbiol Biotechnol 58, 364–369 (2002). https://doi.org/10.1007/s00253-001-0878-3

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  • DOI: https://doi.org/10.1007/s00253-001-0878-3

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