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Immunogenetics

, Volume 53, Issue 1, pp 10–14 | Cite as

Sequence and peptide-binding motif for a variant of HLA-A*0214 (A*02142) in an HIV-1-resistant individual from the Nairobi Sex Worker cohort

  • Mark A. Luscher
  • Kelly S. MacDonald
  • Job J. Bwayo
  • Francis A. Plummer
  • Brian H. Barber
Original Paper

Abstract.

As part of the ongoing study of natural HIV-1 resistance in the women of the Nairobi Sex Workers' study, we have examined a resistance-associated HLA class I allele at the molecular level. Typing by polymerase chain reaction using sequence-specific primers determined that this molecule is closely related to HLA-A*0214, one of a family of HLA-A2 supertype alleles which correlate with HIV-1 resistance in this population. Direct nucleotide sequencing shows that this molecule differs from A*0214, having a silent nucleotide substitution. We therefore propose to designate it HLA-A*02142. We have determined the peptide-binding motif of HLA-A*0214/02142 by peptide elution and bulk Edman degradative sequencing. The resulting motif, X-[Q,V]-X-X-X-K-X-X-[V,L], includes lysine as an anchor at position 6. The data complement available information on the peptide-binding characteristics of this molecule, and will be of use in identifying antigenic peptides from HIV-1 and other pathogens.

HLA-A*0214 Peptide-binding motif HIV-1 resistance African alleles Pool sequence 

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Copyright information

© Springer-Verlag 2001

Authors and Affiliations

  • Mark A. Luscher
    • 2
  • Kelly S. MacDonald
    • 1
  • Job J. Bwayo
    • 3
  • Francis A. Plummer
    • 4
  • Brian H. Barber
    • 5
  1. 1.Department of Microbiology, Mount Sinai Hospital, Room 1484, 600 University Ave., Toronto, Ontario, M5G 1X5, Canada
  2. 2.Department of Medicine, University of Toronto, Toronto, Canada
  3. 3.Department of Microbiology, University of Nairobi, Nairobi, Kenya
  4. 4.Department of Medical Microbiology University of Manitoba, Winnipeg, Canada
  5. 5.Department of Immunology, University of Toronto, Toronto, Canada

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