Identification of the major histocompatibility complex class-II DM and DO alleles in a cohort of northern pig-tailed macaques (Macaca leonina)
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The northern pig-tailed macaque (Macaca leonina) has been considered as an independent species from the pig-tailed macaque group. We have previously reported that this species macaque has the potential to be a useful animal model in HIV/AIDS pathogenesis and vaccine studies due to its susceptibility to HIV-1. To develop this animal into a potential HIV/AIDS model, we have studied the classical MHC genes of this animal. In this study, the non-classical MHC genes Malo-DM and Malo-DO alleles were first characterized by sequencing and cloning in 12 unrelated northern pig-tailed macaques. A total of 20 full-length sequences identified include 4 Malo-DMA, 5 Malo-DMB, 7 Malo-DOA, and 4 Malo-DOB alleles. Most of these allele sequences were shared between northern pig-tailed macaque and other macaque species in exon 2. The full-length MHC-DM and MHC-DO sequences provide more comprehensive analysis of immunogenetics of northern pig-tailed macaques and increase the value of the macaques in further biomedical studies.
KeywordsNorthern pig-tailed macaque Macaca leonina MHC-DM MHC-DO
The authors thank Natasja de Groot, NelOtting, and IMGT Non-Human Primate Nomenclature Committee for naming the Malo-DM and Malo-DO alleles.
Xiao-Dong Lian performed the experiments and wrote the paper; Xiao-Dong Lian, Xi-He Zhang, and Zheng-Xi Dai analyzed the data; and Yong-Tang Zheng conceived and designed the experiments.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
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