Identification of the major histocompatibility complex class-II DM and DO alleles in a cohort of northern pig-tailed macaques (Macaca leonina)
- 110 Downloads
The northern pig-tailed macaque (Macaca leonina) has been considered as an independent species from the pig-tailed macaque group. We have previously reported that this species macaque has the potential to be a useful animal model in HIV/AIDS pathogenesis and vaccine studies due to its susceptibility to HIV-1. To develop this animal into a potential HIV/AIDS model, we have studied the classical MHC genes of this animal. In this study, the non-classical MHC genes Malo-DM and Malo-DO alleles were first characterized by sequencing and cloning in 12 unrelated northern pig-tailed macaques. A total of 20 full-length sequences identified include 4 Malo-DMA, 5 Malo-DMB, 7 Malo-DOA, and 4 Malo-DOB alleles. Most of these allele sequences were shared between northern pig-tailed macaque and other macaque species in exon 2. The full-length MHC-DM and MHC-DO sequences provide more comprehensive analysis of immunogenetics of northern pig-tailed macaques and increase the value of the macaques in further biomedical studies.
KeywordsNorthern pig-tailed macaque Macaca leonina MHC-DM MHC-DO
The authors thank Natasja de Groot, NelOtting, and IMGT Non-Human Primate Nomenclature Committee for naming the Malo-DM and Malo-DO alleles.
Xiao-Dong Lian performed the experiments and wrote the paper; Xiao-Dong Lian, Xi-He Zhang, and Zheng-Xi Dai analyzed the data; and Yong-Tang Zheng conceived and designed the experiments.
This work was supported by the Key Scientific and Technological Program of China (2017ZX10304402-002), the National Basic Research Program of China (2012CBA01305), the National Natural Science Foundation of China (81373124, 81671627, 81172876, 81471620, 81571606), the Knowledge Innovation Program of CAS (ZDRW-ZS-2016-4-2), and the Natural Science Foundation of Yunnan Province (2014FB181, P0120150150).
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
- Hatziioannou T, Del Prete GQ, Keele BF, et al (2014) HIV-1-induced AIDS in monkeys. Science (80-) 344:1401–1405. https://doi.org/10.1126/science.1250761
- Lei AH, Zhang GH, Tian RR et al (2014) Replication potentials of HIV-1/HSIV in PBMCs from northern pig-tailed macaque (Macaca leonina). Zool Res 35:186–195. https://doi.org/10.11813/j.issn.0254-5853.2014.3.186 PubMedPubMedCentralGoogle Scholar
- Liao CH, Kuang YQ, Liu HL et al (2007) A novel fusion gene, TRIM5-cyclophilin A in the pig-tailed macaque determines its susceptibility to HIV-1 infection. AIDS 21(Suppl 8):S19–S26. https://doi.org/10.1097/01.aids.0000304692.09143.1b CrossRefPubMedGoogle Scholar
- Liu B, Jin Y, Lu Y et al (2015) Comprehensive identification of high-frequency and combination MHC-DMA and -DMB alleles in a cohort of Chinese rhesus macaques and cynomolgus macaques of Vietnamese origin. Hum Immunol 76:109–112. https://doi.org/10.1016/j.humimm.2015.01.019 CrossRefPubMedGoogle Scholar
- Pang W, Zhang GH, Jiang J, et al (2017) HIV-1 can infect northern pig-tailed macaques (Macaca leonina) and form viral reservoirs in vivo. Sci Bull (in press). https://doi.org/10.1016/j.scib.2017.09.020
- Zhang MX, Zheng HY, Jiang J et al (2017) Predict disease progression from T cell phenotypes in northern pig-tailed macaques ( Macaca leonina ) during SIVmac239 infection. Immunology. https://doi.org/10.1111/imm.12794