Abstract
The serum collectin mannose-binding lectin (MBL) plays a major role in innate immunity by activation of the lectin complement pathway or by acting as an opsonin. The serum levels of human and animal MBL are associated with susceptibility to a wide range of infections, and the variation of MBL in serum is genetically determined. In the chicken, 14 single nucleotide polymorphisms (SNPs) have so far been found in the MBL promoter region. In this study, the transcription activity of a 670-bp promoter region covering all 14 SNPs from the four MBL promoter alleles A1 to A4 was assessed using a dual-luciferase assay. Of the analysed alleles, A1 showed the highest transcription activity although this allele is frequently found in chickens with low MBL mRNA expression.
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Acknowledgments
This work was supported by the projects “Mapping of transcriptional regulatory elements in the chicken Mannose-Binding Lectin (MBL) promoter” granted by Gluds Legat and “Development of genetic selection technology for polyvalent resistance to infectious diseases” (POLY-REID) (grant number 10-093534) granted by the Danish Council for Strategic Research, the Danish Poultry Council, The Hatchery Hellevad, and Cobb-Vantress, and grants from The Graduate School of Science and Technology at Aarhus University. The authors wish to thank K.V. Østergaard for critical reading of the manuscript. The experimental procedures were conducted under the protocols approved by the Danish Animal Experiments Inspectorate and complied with the Danish Ministry of Justice Law no. 382 (10 June 1987) and Acts 739 (6 December 1988) and 333 (19 May 1990) concerning animal experimentation and care of experimental animals.
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Kjærup, R.M., Dalgaard, T.S., Norup, L.R. et al. Transcription efficiency of different chicken mannose-binding lectin promoter alleles. Immunogenetics 66, 737–742 (2014). https://doi.org/10.1007/s00251-014-0801-6
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DOI: https://doi.org/10.1007/s00251-014-0801-6