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Comprehensive identification of high-frequency and co-occurring Mafa-DPA1, Mafa-DQA1, Mafa-DRA, and Mafa-DOA alleles in Vietnamese cynomolgus macaques

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Abstract

High-frequency alleles and/or co-occurring human leukocyte antigen alleles across loci appear to be more important than individual alleles as markers of disease risk and have clinical value as biomarkers for targeted screening or the development of new disease therapies. To better elucidate the major histocompatibility complex (MHC) background and to facilitate the experimental use of cynomolgus macaques, Mafa-DPA1, Mafa-DQA1, Mafa-DRA, and Mafa-DOA alleles were characterized, and their combinations were investigated in 30 Vietnamese macaques by gene cloning and sequencing. A total of 26 Mafa-DPA1, 18 Mafa-DQA1, 9 Mafa-DRA, and 15 Mafa-DOA alleles, including 7 high-frequency alleles, were identified in this study, respectively. In addition, 15 Mafa-DQA1, 17 Mafa-DPA1, 15 Mafa-DOA, and 2 Mafa-DRA alleles represented novel sequences that had not been documented in earlier studies. Our results also showed that the Vietnamese macaques might be valuable because no less than 30 % of the test animals possessed Mafa-DRA*01:02:01 (90 %), -DQA1*26:01:03 (37 %), -DOA*01:02:07 (34 %), and -DQA1*01:03:03 (30 %). We previously reported that the combinations of MHC class II alleles, including the combination of DOA*01:02:07-DPA1*02:09 and DOA*01:02:07-DQA1*01:03:03, were detected in 17 and 14 % of the animals, respectively. Interestingly, more than two Mafa-DQA1 and Mafa-DPA1 alleles were detected in one animal in this study, which suggested that they might be caused by a chromosomal duplication. If our findings can be validated by other studies, it will further enrich the number of known Mafa-DPA1 and Mafa-DQA1 polymorphisms. Our results identified the co-occurring MHC alleles across loci in a cohort of Vietnamese cynomolgus macaques, which emphasized the value of this species as a model for biomedical research.

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Acknowledgments

We thank the Primate Research Center of South China for providing blood samples from animals. We thank Dr Natasja de Groot, Dr Nel Otting, and IMGT Non-human Primate Nomenclature Committee for naming the Mafa-DPA1, Mafa-DQA1, Mafa-DRA, and Mafa-DOA sequences. This project was granted by the National Natural Science Foundation of China (31271322), the Natural Science Foundation of Guangdong, China (S2011040005261), and the Fundamental Research Funds for the Central Universities of South China University of Technology (2012ZZ0093).

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Authors and Affiliations

  1. School of Bioscience and Bioengineering, South China University of Technology, Guangzhou, 510006, People’s Republic of China

    Ruirui Xiang, Huiling Zhang, Qing Deng, Rui Yue, Haite Tang, Yanqi Zhang, Fei Ling, Min Zhuo, Hongli Du & Xiaoning Wang

  2. Guangdong Landau Biotechnology CO., Ltd., Guangzhou, 510555, People’s Republic of China

    Shiqian Xu & Qiang Xu

  3. School of Life Science, General Hospital of PLA, Beijing, 100853, People’s Republic of China

    Xiaoning Wang

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  1. Ruirui Xiang
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  2. Huiling Zhang
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  3. Qing Deng
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  5. Haite Tang
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  7. Fei Ling
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  8. Min Zhuo
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  9. Hongli Du
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  10. Shiqian Xu
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  11. Qiang Xu
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Correspondence to Fei Ling or Xiaoning Wang.

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Ruirui Xiang and Huiling Zhang contributed equally to this work.

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Xiang, R., Zhang, H., Deng, Q. et al. Comprehensive identification of high-frequency and co-occurring Mafa-DPA1, Mafa-DQA1, Mafa-DRA, and Mafa-DOA alleles in Vietnamese cynomolgus macaques. Immunogenetics 65, 667–674 (2013). https://doi.org/10.1007/s00251-013-0713-x

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