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Cloning of a hamster anti-mouse CD79B antibody sequences and identification of a new hamster immunoglobulin lambda constant IGLC gene region

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Abstract

Anti-CD79 antibodies have been effective at targeting B cell lymphoma cells and depleting B cells in animal models. In order to engineer recombinant antibodies with additional effector functions in mice, we cloned and sequenced the full-length cDNAs of the heavy and light chain of a hamster anti-mouse CD79B antibody. Although hamster antibodies represent a unique source of monoclonal antibodies against mouse, rat, and human antigens, sequence information of hamster immunoglobulins (IG) is sparse. Here, we report a new hamster (Cricetulus migratorius) IG lambda constant (IGLC) gene region that is most homologous to mouse IGLC2 and IGLC3.

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Acknowledgments

We thank Dr. Robert Eisenberg for providing the HM79-16 hybridoma and the University of Chicago Comprehensive Cancer Center DNA Sequencing and Genotyping Facility for sequencing. This work was supported by a NIH NIAID grant R01-AI087645 (H. Huang). Jason Perera was partially supported by NIH/NIAID grant 2T32AI007090.

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Authors and Affiliations

  1. Department of Medicine, Section of Rheumatology, Knapp Center for Lupus and Immunology Research, University of Chicago, Chicago, IL, 60637, USA

    Ryan Haggart, Jason Perera & Haochu Huang

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  1. Ryan Haggart
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  2. Jason Perera
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  3. Haochu Huang
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Correspondence to Haochu Huang.

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Haggart, R., Perera, J. & Huang, H. Cloning of a hamster anti-mouse CD79B antibody sequences and identification of a new hamster immunoglobulin lambda constant IGLC gene region. Immunogenetics 65, 473–478 (2013). https://doi.org/10.1007/s00251-013-0698-5

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  • DOI: https://doi.org/10.1007/s00251-013-0698-5

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