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Meiotic recombinations within major histocompatibility complex of human embryos

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Abstract

We studied the rate and pattern of recombinations within the extended major histocompatibility complex (MHC) locus of the human embryos obtained during preimplantation genetic diagnosis (PGD) for HLA compatibility. Recombinant allele frequency was on average 5.33 %, and recombination rate was 0.44 cM/Mb in the 12.2 Mb of the extended MHC locus. Recombination rate varied up to 14-fold (0.19–2.73 cM/Mb) between cases, and maternal recombination rate was on average 3.8 times higher than paternal alleles. More than 69 % of the recombination hot spots were clustered within the extended class II region where the recombination rate was 5.4 times more than that in extended class I region. These findings indicate the potential of PGD to study the mechanisms of linkage disequilibrium within MHC locus of human embryos, demonstrate the recombination characteristics within extended MHC loci of human embryos in comparison to sperm and family studies, and point to the significance of design and interpretation of PGD for HLA compatibility to avoid misdiagnosis because of meiotic recombinations.

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Acknowledgments

We thank Dr. Engin Oral and Dr. Necati Findikli at Istanbul International Hospital for providing IVF embryos.

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  1. Fulya Taylan & Ender Altiok

    Present address: Department of Genetics and Bioengineering, Okan University, Tuzla, Istanbul, 34959, Turkey

Authors and Affiliations

  1. Department of Molecular Biology and Genetics, Bogazici University, Istanbul, Turkey

    Fulya Taylan & Ender Altiok

  2. Acibadem Genetic Diagnosis Center, Istanbul, Turkey

    Fulya Taylan & Ender Altiok

  3. Department of Medical Biology, Faculty of Medicine, Acibadem University, Istanbul, Turkey

    Ender Altiok

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  1. Fulya Taylan
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Correspondence to Ender Altiok.

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Taylan, F., Altiok, E. Meiotic recombinations within major histocompatibility complex of human embryos. Immunogenetics 64, 839–844 (2012). https://doi.org/10.1007/s00251-012-0644-y

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