Non-classical MHC-Ι genes in chronic hepatitis B and hepatocellular carcinoma
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The non-classical human leukocyte antigens (HLA)-E, HLA-G, and HLA-F have been shown to modulate immune responses. We examined whether non-classical HLA polymorphisms are associated with hepatitis B virus (HBV) infection and hepatocellular carcinoma (HCC). Fifteen Single-nucleotide polymorphisms (SNPs) in these non-classical class I alleles were investigated by ligase detection reaction. A fragment of 650 bp located in the 3' untranslated region of HLA-G was investigated. Four SNPs (rs17875380, rs41557518, rs114465251, and rs115492845) were associated with altered susceptibility to HBV or HCC, and HLA-F*01:04, HLA-G*01:05N, and HLA-E*01:01 were associated with hepatitis B or hepatitis B complicated with HCC. Six of 16 designated HLA-E, -G, and -F haplotypes were associated with risk of hepatitis B or HCC. Our study provides healthy reference and detailed analyses of non-classical HLA class Ι polymorphisms that provide insight into immune mechanisms involved in susceptibility to hepatitis B and HCC.
KeywordsHepatitis B virus Hepatocellular carcinoma Major histocompatibility complex Single nucleotide polymorphism 3' Untranslated region
Human leukocyte antigen
Hepatitis B virus
Hepatitis C virus
Ligase detection reaction
Major histocompatibility complex
- Indel (Ins/Del
Graft versus host disease
This study was supported by the Key Program of National Natural Science Foundation of China (no. 30730085), Scientific Research Fund of Zhejiang Provincial Education Department grant (no. Y200908541), and the National Basic Research Program of China (973 Program) (no. 2009CB522403). We thank Professor Martin F. Flajnik, Haiyang Xie, and Xiaobo Yu for their valuable assistance.
Conflict of interest
The authors declare no conflict of interest.
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