, Volume 64, Issue 3, pp 251–258 | Cite as

Non-classical MHC-Ι genes in chronic hepatitis B and hepatocellular carcinoma

  • Jian Zhang
  • Lelin Pan
  • Luyan Chen
  • Xiaowen Feng
  • Lin Zhou
  • Shusen ZhengEmail author
Brief Communication


The non-classical human leukocyte antigens (HLA)-E, HLA-G, and HLA-F have been shown to modulate immune responses. We examined whether non-classical HLA polymorphisms are associated with hepatitis B virus (HBV) infection and hepatocellular carcinoma (HCC). Fifteen Single-nucleotide polymorphisms (SNPs) in these non-classical class I alleles were investigated by ligase detection reaction. A fragment of 650 bp located in the 3' untranslated region of HLA-G was investigated. Four SNPs (rs17875380, rs41557518, rs114465251, and rs115492845) were associated with altered susceptibility to HBV or HCC, and HLA-F*01:04, HLA-G*01:05N, and HLA-E*01:01 were associated with hepatitis B or hepatitis B complicated with HCC. Six of 16 designated HLA-E, -G, and -F haplotypes were associated with risk of hepatitis B or HCC. Our study provides healthy reference and detailed analyses of non-classical HLA class Ι polymorphisms that provide insight into immune mechanisms involved in susceptibility to hepatitis B and HCC.


Hepatitis B virus Hepatocellular carcinoma Major histocompatibility complex Single nucleotide polymorphism 3' Untranslated region 



Human leukocyte antigen


Hepatitis B virus


Hepatitis C virus


Untranslated region


Hepatocellular carcinoma


Ligase detection reaction


Single-nucleotide polymorphism


Linkage disequilibrium


Major histocompatibility complex

Indel (Ins/Del

I/D), Insertion/deletion


Log odds


Graft versus host disease



This study was supported by the Key Program of National Natural Science Foundation of China (no. 30730085), Scientific Research Fund of Zhejiang Provincial Education Department grant (no. Y200908541), and the National Basic Research Program of China (973 Program) (no. 2009CB522403). We thank Professor Martin F. Flajnik, Haiyang Xie, and Xiaobo Yu for their valuable assistance.

Conflict of interest

The authors declare no conflict of interest.

Supplementary material

251_2011_580_MOESM1_ESM.doc (104 kb)
ESM 1 (DOC 104 kb)


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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Jian Zhang
    • 1
  • Lelin Pan
    • 1
  • Luyan Chen
    • 1
  • Xiaowen Feng
    • 1
  • Lin Zhou
    • 1
  • Shusen Zheng
    • 1
    Email author
  1. 1.Key Laboratory of Combined Multi-organ Transplantation, Ministry of Public HealthThe First Affiliated Hospital, School of Medicine Zhejiang UniversityHangzhouPeople’s Republic of China

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