Immunogenetics

, Volume 63, Issue 10, pp 653–666 | Cite as

Characterization of MHC class I and II genes in a subantarctic seabird, the blue petrel, Halobaena caerulea (Procellariiformes)

  • Maria Strandh
  • Mimi Lannefors
  • Francesco Bonadonna
  • Helena Westerdahl
Original Paper

Abstract

The great polymorphism observed in the major histocompatibility complex (MHC) genes is thought to be maintained by pathogen-mediated selection possibly combined with MHC-disassortative mating, guided by MHC-determined olfactory cues. Here, we partly characterize the MHC class I and II B of the blue petrel, Halobaena caerulea (Procellariiformes), a bird with significant olfactory abilities that lives under presumably low pathogen burdens in Subantarctica. Blue petrels are long-lived, monogamous birds which suggest the necessity of an accurate mate choice process. The species is ancestral to songbirds (Passeriformes; many MHC loci), although not to gamefowls (Galliformes; few MHC loci). Considering the phylogenetic relationships and the low subantarctic pathogen burden, we expected few rather than many MHC loci in the blue petrel. However, when we analysed partial MHC class I and class II B cDNA and gDNA sequences we found evidence for as many as at least eight MHC class I loci and at least two class II B loci. These class I and II B sequences showed classical MHC characteristics, e.g. high nucleotide diversity, especially in putative peptide-binding regions where signatures of positive selection was detected. Trans-species polymorphism was found between MHC class II B sequences of the blue petrel and those of thin-billed prion, Pachyptila belcheri, two species that diverged ∼25 MYA. The observed MHC allele richness in the blue petrel may well serve as a basis for mate choice, especially since olfactory discrimination of MHC types may be possible in this species.

Keywords

Major histocompatibility complex Class I Class II B Diversity Selection Aves 

Supplementary material

251_2011_534_MOESM1_ESM.pdf (387 kb)
Supplementary Fig. 1Phylogenetic tree of major histocompatibility complex class I exons 2–4 translated sequences (Haca-UA*xxL, GenBank accession numbers, Acc nr JF276877–JF276884) from the blue petrel, Halobaena caerulea, five other bird species (Acc nr and species codes are given: Grca Grus canadensis pratensis, Anan Anser anser, Anpl Anas platyrhynchos, Gaga Gallus gallus and Acar Acrocephalus arundinaceus). Human HLA-A (Hosa_NM002116) was used as outgroup. Neighbour-joining bootstrap consensus tree from 2,000 replicates (Felsenstein 1985; Saitou and Nei 1987) constructed in MEGA4 (Tamura et al. 2007). Filled squares/circles represent sequences from each of two blue petrel individuals (PDF 386 kb)
251_2011_534_MOESM2_ESM.pdf (34 kb)
Supplementary Fig. 2Amino acid alignment of major histocompatibility complex class I exon 3 translated sequences (Haca-UA*01-Haca-UA*45, Acc nr JF276832–JF276876) from the blue petrel, Halobaena caerulea, five other bird species (Acc nr and species codes are given: Grca Grus canadensis pratensis, Anan Anser anser, Anpl Anas platyrhynchos, Gaga Gallus gallus, Acar Acrocephalus arundinaceus) and human HLA-A (Hosa_NM002116). Peptide-binding regions were inferred (a pocket a, f pocket f, c other peptide contacts, t t-cell contacts) (Wallny et al. 2006). Positively selected amino acid sites (plus sign) identified with model 8 in CODEML, PAML (Yang 2007) (PDF 33 kb)
251_2011_534_MOESM3_ESM.pdf (323 kb)
Supplementary Fig. 3Southern blots of major histocompatibility complex (MHC) probes hybridized to Pvu II cleaved genomic DNA from four individuals of blue petrels, Halobaena caerulea. Each lane represents an individual (1–4). The left panel shows a MHC class I exon 3 probed blot and the right panel a MHC class II B exon 2 probed blot of the same four individuals (PDF 323 kb)
251_2011_534_MOESM4_ESM.pdf (32 kb)
Supplementary Fig. 4Amino acid alignment of major histocompatibility complex class II B exon 2 translated sequences (Haca-DAB*01-Haca-DAB*08, Acc nr JF276885–JF276892) from the blue petrel, Halobaena caerulea, and the thin-billed prion, Pachyptila belcheri (Pabe, Acc nr are given). Peptide-binding regions were inferred (c peptide contacts) (Brown et al. 1993). Positively selected amino acid sites (plus sign) identified with model 8 in CODEML, PAML (Yang 2007) (PDF 31 kb)
251_2011_534_MOESM5_ESM.doc (158 kb)
Supplementary Table 1Major histocompatibility alleles (class I Haca-UA, class II B Haca-DAB, L long sequences, GenBank Acc nr JF276832–JF276894) identified by sequencing MHC clones from 25 blue petrel, Halobaena caerulea, individuals (1–25; g genomic DNA, c cDNA) (DOC 157 kb)

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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Maria Strandh
    • 1
    • 2
  • Mimi Lannefors
    • 2
  • Francesco Bonadonna
    • 1
  • Helena Westerdahl
    • 2
  1. 1.Behavioral Ecology GroupCEFE-CNRSMontpellier Cedex 5France
  2. 2.Molecular Ecology and Evolution LabLund universityLundSweden

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