Abstract
We set up a cohort of HIV-infected, asymptomatic Japanese patients with hemophilia for follow-up study in 1995. All subjects who had been infected with HIV-1 for more than 10 years met the criteria for long-term nonprogressors (LTNPs) at the time of entry; however, some of them later developed lymphopenia and required antiretroviral treatment during five more years of observation. In this study, we investigated the impacts of the CCL3L1 dose on the long-term prognosis in the subjects with chronic HIV-1 infection. We collected genomic DNA from 95 long-term survivors including 48 nonprogressors and 47 subjects receiving antiretroviral treatment. The distributions of CCL3L1 copy number significantly differed between the 95 HIV-1-infected subjects with hemophilia and 205 controls. Average copy number of CCL3L1 in the HIV-1-infected subjects was significantly lower than in control (5.00 ± 0.22 vs 3.35 ± 0.24, p < 0.001). Moreover, the subjects possessing two or less copies of CCL3L1 had significantly higher risk of acquiring HIV-1. However, CCL3L1 copy number variations had no significant effect on the disease progression among the LTNP subjects who had been afflicted with chronic HIV-1 infection for more than 15 years, when compared between nonprogressors and patients under treatment (3.68 ± 0.37 vs 3.02 ± 0.29, ns). Furthermore, variations in the CCL3L1 copy number had little effect on the levels of HIV-1 load among them. We conclude that variation in the CCL3L1 copy number is apparently not a factor that determines the prognosis of chronic HIV-1 infection, even though it is linked to HIV-1 susceptibility.
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Acknowledgements
We are grateful for samples of HIV-1-positive patients to Drs. H. Hanabusa (Ogikubo Hospital), J. Matsuda (Teikyo University School of Medicine), M. Sakai (University of Occupational and Environmental Health), S. Ikeda (Sasebo Municipal Hospital), and T. Fujii (Hiroshima University School of Medicine). This work was supported in part by research grants from the Ministry of Health, Labor and Welfare, Japan, the Japan Health Science Foundation, and the program of Founding Research Centers for Emerging and Reemerging Infection Disease supported by the Ministry of Education, Culture, Sports, Science, and Technology, Japan.
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Nakajima, T., Ohtani, H., Naruse, T. et al. Copy number variations of CCL3L1 and long-term prognosis of HIV-1 infection in asymptomatic HIV-infected Japanese with hemophilia. Immunogenetics 59, 793–798 (2007). https://doi.org/10.1007/s00251-007-0252-4
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DOI: https://doi.org/10.1007/s00251-007-0252-4