Abstract
The human TNF-alpha gene is characterized by several single nucleotide polymorphisms (SNPs) in its promoter region, in part having been shown to influence TNF-alpha expression and susceptibility to various diseases. The rhesus macaque is widely used as an animal model for a variety of TNF-alpha associated pathological conditions, but little is known about genetic variation within the TNF-alpha promoter region. In order to check for such polymorphisms, primers based on rhesus sequence within 5′ UTR were designed and used to amplify a ∼1 kb product from genomic DNA of 29 animals. Sequencing and cloning revealed a total of 11 polymorphisms leading to five different haplotypes.
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Acknowledgements
This work was supported by NIH grant RR0340 to the Caribbean Primate Research Center, University of Puerto Rico Medical Sciences Campus.
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Singh, K.K., Schmidtke, J. Single nucleotide polymorphisms within the promoter region of the rhesus monkey tumor necrosis factor-alpha gene. Immunogenetics 57, 289–292 (2005). https://doi.org/10.1007/s00251-005-0799-x
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DOI: https://doi.org/10.1007/s00251-005-0799-x