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TRAV gene usage in pig T-cell receptor alpha cDNA

Immunogenetics volume 57, pages 219–225 (2005)Cite this article

Abstract

Pig (Sus scrofa) TRA clones were isolated from cDNA libraries of total RNA from two different sources, the thymus of a 1-month-old LW strain pig and the peripheral blood lymphocytes of a 5-month-old Clawn strain pig. Among 103 complete TRA cDNA clones from both sources, 33 different TRAV genes were identified. By comparing their sequence identities against one another, these pig TRAV genes were grouped into 20 subgroups, including 13 subgroups, each containing only a single member. All of these pig subgroups gave corresponding human and mouse functional counterparts, suggesting their functional commonality. An exception was the Va01 gene segment, which lacked a functional human counterpart. The present report provides groundwork for studies on pig TRA expression.

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Acknowledgements

We thank Tomiji Akita for care of pigs. This work was supported by grants from National Institute of Agrobiological Sciences, Japan.

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Affiliations

  1. Genome Research Department, National Institute of Agrobiological Sciences, 2 Ikenodai, Tsukuba, 305-8602, Japan

    Ryuji Yamamoto, Hirohide Uenishi, Takashi Awata & Hiroshi Yasue

  2. Department of Molecular Genetics, Graduate School of Medical Sciences, Kitasato University, 1-15-1 Kitasato, Sagamihara, 228-8555, Japan

    Ryuji Yamamoto, Hiromi Hatsuse & Yohtaroh Takagaki

  3. Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumi-dori Amamiya-machi, Aoba, Sendai, 981-8555, Japan

    Ryuji Yamamoto & Eimei Sato

Authors
  1. Ryuji Yamamoto
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  2. Hirohide Uenishi
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  3. Hiromi Hatsuse
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  4. Eimei Sato
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  5. Takashi Awata
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  6. Hiroshi Yasue
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  7. Yohtaroh Takagaki
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Corresponding author

Correspondence to Yohtaroh Takagaki.

Additional information

This report constitutes a part of the requirement for the doctorate thesis for R.Y. in Tohoku University.

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Yamamoto, R., Uenishi, H., Hatsuse, H. et al. TRAV gene usage in pig T-cell receptor alpha cDNA. Immunogenetics 57, 219–225 (2005). https://doi.org/10.1007/s00251-005-0779-1

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  • DOI: https://doi.org/10.1007/s00251-005-0779-1

Keywords

  • T-cell receptor alpha (TRA) chain
  • Pig