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Characterization and expression analysis of CD3ɛ and CD3γ/δ in fugu, Takifugu rubripes

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Abstract

CD3 is an essential component of the CD3-TCR complex. In this report, we describe the cloning, characterization, and expression analysis of the CD3ɛ and CD3γ/δ chain genes from fugu, Takifugu rubripes. Two distinct CD3ɛ homologue cDNAs, designated as CD3ɛ-1 and CD3ɛ-2, and a CD3γ/δ homologue cDNA were isolated from the fugu thymus. The deduced amino acid sequences of these cDNAs exhibit conserved essential CD3 chain motifs and overall structures. RT-PCR analysis demonstrated that the CD3ɛ and CD3γ/δ genes were expressed in lymphoid organs (e.g. thymus, head kidney, trunk kidney and spleen), mucosal tissues (gill, skin, and intestine), and peripheral blood leucocytes (PBL). The CD3 and TCRα genes were expressed only in the surface IgM− population, which were separated from PBL using an anti-fugu IgM monoclonal antibody. In addition, in situ hybridization confirmed that CD3-expressing cells were distributed randomly in the head kidney, trunk kidney, and spleen, but in the thymus were restricted to the lymphoid outer zone and epithelioid inner zone only. Collectively, these results suggest that CD3 molecules are useful markers for the identification of T cells in teleost fish. The present study thus provides a critical step in identifying T cells in this model organism.

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Acknowledgements

We would like to thank the staff of Fukui Prefecture Fisheries Experimental Station for the provision of fugu. The authors thank Dr. T. Miyadai, Fukui Prefectural University, Japan, for supplying us with the anti-IgM Mab 4H5. This work was supported by a Grant-in-Aid for Creative Basic Research 12NP0201 from the Ministry of Education, Science, Sports and Culture of Japan.

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Authors and Affiliations

  1. Fisheries Laboratory, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 2971-4 Maisaka, Shizuoka, 431-0211, Japan

    Kyosuke Araki, Hiroaki Suetake, Kiyoshi Kikuchi & Yuzuru Suzuki

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  1. Kyosuke Araki
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  2. Hiroaki Suetake
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  3. Kiyoshi Kikuchi
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  4. Yuzuru Suzuki
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Corresponding author

Correspondence to Hiroaki Suetake.

Additional information

Nucleotide sequence data reported in this paper are available in the DBJ/EMBL/GenBank databases and have been assigned the accession numbers AB166798 (CD3ɛ-1), AB166799 (CD3ɛ-2), and AB166800 (CD3γ/δ).

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Araki, K., Suetake, H., Kikuchi, K. et al. Characterization and expression analysis of CD3ɛ and CD3γ/δ in fugu, Takifugu rubripes. Immunogenetics 57, 158–163 (2005). https://doi.org/10.1007/s00251-005-0772-8

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  • DOI: https://doi.org/10.1007/s00251-005-0772-8

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