Abstract
The seventh component of complement is a single-chain plasma glycoprotein that is involved in the cytolytic phase of complement activation through a sequence of polymerization reactions with other terminal components. We have previously isolated and characterized a C7 gene in rainbow trout (Oncorhynchus mykiss). Here, we report the cloning of a second trout C7 gene (C7-2). The deduced amino acid sequence of the C7-2 gene exhibits 43 and 50% identity with human C7 and trout C7-1, respectively. The structural motifs of trout C7-2 resemble those of mammalian C7 more than trout C7-1, and the cysteine backbone shows a high degree of conservation. C7-2 presents a different tissue expression profile from trout C7-1, which correlates with that of mammalian counterparts. Although duplication of complement genes is a common observation in teleost fish, this is the first report of two gene isotypes of a terminal membrane attack complex/perforin complement component in any organism.
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Acknowledgements
We are grateful to Dr. D. Mastellos for critically reading the manuscript. We thank the European Social Fund (ESF), Operational Program for Educational and Vocational Training II (EPEAEK II), and, particularly, the Program PYTHAGORAS for funding the above work. The sequence described in this paper has been deposited in the EMBL database under accession number AJ622902 (trout C7-2).
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Papanastasiou, A.D., Zarkadis, I.K. Gene duplication of the seventh component of complement in rainbow trout. Immunogenetics 57, 703–708 (2005). https://doi.org/10.1007/s00251-005-0028-7
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DOI: https://doi.org/10.1007/s00251-005-0028-7