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Characterization of multiple alleles of the T-cell differentiation marker ART2 (RT6) in inbred and wild rats

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Abstract

ART2 (RT6) belongs to the family of mono-ADP-ribosyltransferases (ARTs). ART2 is a T-cell differentiation marker expressed by the majority of mature peripheral T cells in the rat. The two known ART2 allotypes display approximately 95% amino acid identity. We sequenced the ART2 coding regions from 18 inbred rat strains and found two additional alleles, termed Art2 a2 and Art2 b2. Monoclonal antibody Gy12/61 specifically reacted with Art2 a2 but not Art2 a1 lymph node cells. Expression of ART2 allotypes in Jurkat cells confirmed this specificity. A polymerase chain reaction (PCR) assay using restriction fragment length polymorphisms is described, which allows the easy discrimination of Art2 alleles. All four laboratory rat alleles, as well as an additional sequence variant, were found amongst 18 wild rat DNA samples. PCR analysis confirmed the selective presence of a rodent identifier (ID) element in the Art2 a but not the Art2 b alleles in all rats studied. Analysis of Art2 a1 and Art2 b2 genes showed greater divergence in coding than in non-coding regions. Together with the finding of a high number of non-synonymous mutations leading mostly to non-conservative amino acid substitutions clustered on the side facing away from the cell surface, this suggests that the Art2 polymorphism has been subject to selection.

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Acknowledgements

We thank Mr. Luke Mercer for his help in obtaining the UK rat DNA samples. This work was supported by a stipend of the Werner-Otto Foundation to SR and UK BBSRC CSG funding to GWB's laboratory.

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Authors and Affiliations

  1. Signal Transduction Group, Institute for Immunology, H23W, Universitäts-Klinikum Eppendorf, Martinistr. 52, 20246, Hamburg, Germany

    Stefan Rothenburg, Friedrich Haag & Friedrich Koch-Nolte

  2. Babraham Hall, The Babraham Institute, Cambridge, CB2 4AT, UK

    Christine Carter, Margaret Graham & Geoffrey W. Butcher

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  1. Stefan Rothenburg
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  2. Friedrich Haag
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  3. Friedrich Koch-Nolte
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  4. Christine Carter
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  5. Margaret Graham
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  6. Geoffrey W. Butcher
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Correspondence to Stefan Rothenburg.

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Rothenburg, S., Haag, F., Koch-Nolte, F. et al. Characterization of multiple alleles of the T-cell differentiation marker ART2 (RT6) in inbred and wild rats. Immunogenetics 57, 739–749 (2005). https://doi.org/10.1007/s00251-005-0026-9

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  • DOI: https://doi.org/10.1007/s00251-005-0026-9

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