Skip to main content
SpringerLink
Log in

Rat T-cell receptor TRAV11 (Vα14) genes: further evidence of extensive multiplicity with homogeneous CDR1 and diversified CDR2 by genomic contig and cDNA analysis

  • Original Paper
  • Published:
Immunogenetics Aims and scope Submit manuscript

Abstract

The antigen recognition system of NKT cells acts via an invariant T-cell receptor (TR) which recognizes CD1d and is highly conserved in mice, rats and humans. NKT cells expressing an invariant mouse TR composed of TRAV11-TRAJ18 (formerly Vα14-Jα281) are positively selected by CD1d, and recognize an antigen in context with CD1d. Here we show ten distinct TRAV11 genes (previously designated by us as TRAV14) on rat Chromosome 15 (BN/SsNHsd/MCW strain). In the rat TRAV11 genes, the splicing sites, the recombination signal sequences, and the possible promoter regions were well conserved, indicating that they were functional. Predicted protein sequences of rat TRAV11 genes were analyzed, including the three loops (CDR1–3) which connect the β-strands of the domain encoded by the TRA V-REGION and is hypervariable in sequence. The CDR1-IMGT sequence (from 27 to 32; VTPFNN) was conserved among most rat TRAV11 genes. The CDR2-IMGT sequences (from 56 to 61) were grouped into two types: type 1 [L(T/K)NKEE], and type 2 [LAYKKE]. The mRNAs of both types have a different tissue distribution. The CDR3 sequences were short and invariant, the rat TRAV11 genes being preferentially rearranged with rat TRAJ18 (Jα281), with the joint consisting of a single amino acid (A or G). Thus, rats had multiple TRAV11 chains with diversified CDR2-IMGT and homogenous CDR1-IMGT and CDR3-IMGT.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  • Bendelac A, Lantz O, Quimby ME, Tewdell JW, Bennink J, Brutkiewicz RR (1995) CD1 recognition by mouse NK1+ T lymphocytes. Science 268:863–265

    CAS  PubMed  Google Scholar 

  • Bosc N, Lefranc MP (2003) The mouse (Mus musculus) T-cell receptor alpha (TRA) and delta (TRD) variable genes. Dev Comp Immunol 27:465–497

    Article  CAS  PubMed  Google Scholar 

  • Bouwens L, Remels L, Baekeland M, Van Bossuyt H, Wisse E (1987) Large granular lymphocytes or “pit cells” from rat liver: isolation, ultrastructural characterization and natural killer activity. Eur J Immunol 17:37–42

    PubMed  Google Scholar 

  • Chothia C, Boswell DR, Lesk AM (1988) The outline structure of the T-cell alpha beta receptor. EMBO J 7:3745–3755

    CAS  PubMed  Google Scholar 

  • Chou HS, Behlke MA, Godambe SA, Russell JH, Brooks CG, Loh DY (1986) T-cell receptor genes in an alloreactive CTL clone: implications for rearrangement and germline diversity of variable gene segments. EMBO J 5:2149–2155

    CAS  PubMed  Google Scholar 

  • Clark SP, Arden B, Kabelitz D, Mak TW (1995) Comparison of human and mouse T-cell receptor variable gene segment subfamilies. Immunogenetics 42:531–540

    Google Scholar 

  • Frohman MA, Dush M, Martin GR (1988) Rapid production of full-length cDNAs from rare transcripts: amplification using a single gene-specific oligonucleotide primer. Proc Natl Acad Sci USA 85:8998–9002

    PubMed  Google Scholar 

  • Gahm SJ, Fowlkes BJ, Jameson SC, Gascoigne NR, Cotterman MM, Kanagawa O, Schwartz RH, Matis LA (1991) Profound alteration in an alpha beta T-cell antigen receptor repertoire due to polymorphism in the first complementarity-determining region of the beta chain. Proc Natl Acad Sci USA 88:10267–10271

    CAS  PubMed  Google Scholar 

  • Hesse JE, Lieber MR, Mizuuchi K, Gellert M (1989) V(D)J recombination: a functional definition of the joining signals. Genes Dev 3:1053–1061

    CAS  PubMed  Google Scholar 

  • Holder M, Lewis PO (2003) Phylogeny estimation: traditional and Bayesian approaches. Nat Rev Genet 4:275–284

    Article  CAS  PubMed  Google Scholar 

  • Jameson SC, Nakajima PB, Brooks JL, Heath W, Kanagawa O, Gascoigne NR (1991) The T-cell receptor V alpha 11 gene family. Analysis of allelic sequence polymorphism and demonstration of J alpha region-dependent recognition by allele-specific antibodies. J Immunol 147:3185–3193

    CAS  PubMed  Google Scholar 

  • Kinebuchi M, Matsuura A, Ogiu T, Kikuchi K (1997) Deviated overexpression of TCR-beta, TCR-gamma, CD4, and CD8 on thymic lymphomas induced by 1-propyl-1-nitrosourea: destruction of the allelic exclusion of TCR-beta and expression of functional TCR-betagamma heterodimer on a lymphoma, cFTL53. J Immunol 159:748–756

    CAS  PubMed  Google Scholar 

  • Koseki H, Imai K, Nakayama F, Sado T, Moriwaki K, Taniguchi M (1990) Homogenous junctional sequence of the V14+ T-cell antigen receptor alpha chain expanded in unprimed mice. Proc Natl Acad Sci USA 87:5248–5252

    CAS  Google Scholar 

  • Koseki H, Asano H, Inaba T, Miyashita N, Moriwaki K, Lindahl KF, Mizutani Y, Imai K, Taniguchi M (1991) Dominant expression of a distinctive V14+ T-cell antigen receptor alpha chain in mice. Proc Natl Acad of Sci USA 88:7518–7522

    CAS  Google Scholar 

  • Lefranc M-P, Lefranc G (2001) The T-cell receptor facts book. Academic Press, London

  • Lefranc MP, Pommie C, Ruiz M, Giudicelli V, Foulquier E, Truong L, Thouvenin-Contet V, Lefranc G (2003) IMGT unique numbering for immunoglobulin and T cell receptor variable domains and Ig superfamily V-like domains. Dev Comp Immunol 27:55–77

    Article  CAS  PubMed  Google Scholar 

  • Lewin B (2000) Genes VII. Oxford University Press, Oxford

  • Lipman DJ, Pearson WR (1985) Rapid and sensitive protein similarity searches. Science 227:1435–1441

    CAS  PubMed  Google Scholar 

  • Matsuura A, Kinebuchi M, Chen HZ, Katabami S, Shimizu T, Hashimoto Y, Kikuchi K, Sato N (2000) NKT cells in the rat: organ-specific distribution of NK T cells expressing distinct V alpha 14 chains. J Immunol 164:3140–3148

    CAS  PubMed  Google Scholar 

  • Morris M, Barclay AN, Williams AF (1988) Analysis of T-cell receptor beta chains in rat thymus, and rat C alpha and C beta sequences. Immunogenetics 27:174–179

    CAS  PubMed  Google Scholar 

  • Nei M, Gojobori T (1986) Simple methods for estimating the numbers of synonymous and nonsynonymous nucleotide substitutions. Mol Biol Evol 3:418–426

    CAS  PubMed  Google Scholar 

  • Sanger F, Nicklen S, Coulson AR (1977) DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74:5463-5467

    PubMed  Google Scholar 

  • Taniguchi M, Harada M, Kojo S, Nakayama T, Wakao H (2003) The regulatory role of Valpha14 NKT cells in innate and acquired immune response. Annu Rev Immunol 21:483–513

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Pathology, School of Medicine, Fujita Health University, Toyoake, Aichi, Japan

    Miyuki Kinebuchi & Akihiro Matsuura

Authors
  1. Miyuki Kinebuchi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Akihiro Matsuura
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Miyuki Kinebuchi.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kinebuchi, M., Matsuura, A. Rat T-cell receptor TRAV11 (Vα14) genes: further evidence of extensive multiplicity with homogeneous CDR1 and diversified CDR2 by genomic contig and cDNA analysis. Immunogenetics 55, 756–762 (2004). https://doi.org/10.1007/s00251-003-0640-3

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00251-003-0640-3

Keywords

Search

Navigation