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Analysis of part of the chicken Rfp-Y region reveals two novel lectin genes, the first complete genomic sequence of a class I α-chain gene, a truncated class II β-chain gene, and a large CR1 repeat

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Abstract

The Rfp-Y region lies on the same microchromosome as the B-F/B-L region of the B complex, yet in contrast to the latter it is poorly characterised. To date it has been shown to contain at least two class I α-chain (Y-F) genes, a class II B-chain gene and a C-type lectin-like gene. We describe the sequencing and analysis of some 20 kb of the Rfp-Y region, and identify several new genes. These include two novel C-type lectin-like genes (Y-Lec1 and Y-Lec2) that differ strongly from the previously described C-type lectin-like gene found in the Rfp-Y region. We describe a complete genomic sequence of a class I α-chain (Y-F) gene and its promoter from the Rfp-Y region. The predicted cDNA from this gene has high homology to the previously reported Y-F cDNAs. The promoter contains an altered enhancer A element. This portion of the Rfp-Y region also contains a truncated class II B-chain (Y-LB) gene, as well as a large chicken repeat 1 (CR1) element.

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Acknowledgements

This research was undertaken with the financial support of the Biotechnology and Biological Sciences Research Council. The experiments comply with the current laws of the UK.

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  1. Iain Shaw

    Present address: Immunodiagnostics Laboratory, National Diagnostics Centre, National University of Ireland, Galway, Eire

Authors and Affiliations

  1. Institute for Animal Health, Compton, Berkshire, RG20 7NN, UK

    Sally Rogers, Iain Shaw, Norman Ross, Venugopal Nair, Lisa Rothwell, Jim Kaufman & Pete Kaiser

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  1. Sally Rogers
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Correspondence to Pete Kaiser.

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Rogers, S., Shaw, I., Ross, N. et al. Analysis of part of the chicken Rfp-Y region reveals two novel lectin genes, the first complete genomic sequence of a class I α-chain gene, a truncated class II β-chain gene, and a large CR1 repeat. Immunogenetics 55, 100–108 (2003). https://doi.org/10.1007/s00251-003-0553-1

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