Membrane transport of polysialic acid chains: modulation of transmembrane potential

Abstract

Polysialic acid (polySia) is a long polyanionic polymer (with the degree of polymerization, DP, up to 200) of negatively charged sialic acid monomers. PolySia chains are bound to the external surface of some neuroinvasive bacterial cells and neural cells. PolySia serves as a potent regulator of cell interactions via its unusual biophysical properties. In the present paper, the analysis, based on the Goldman-Hodgkin-Katz equation, of transmembrane potential changes resulting from transmembrane translocation of polySia is performed. The relationships between the transmembrane potential and the polySia DP (up to 200), the temperature, the cation/anion permeability ratio, and the inner/outer concentration ratio of polySia has been plotted and discussed. The maximal membrane potential changes, up to 118 mV, were found for a permeability ratio greater than one. The increase of the polySia chain length resulted in the diminution of this effect. The temperature-dependent changes in membrane potential were less than 7 mV in the range 0–50 °C. The change in the concentration ratio (into its reciprocal) resulted in a mirror reflection of the membrane potential curves. The results show that the expression of polySia chains in bacterial cells can be responsible for the modulation of the transmembrane potential of the bacterial inner membrane. We suggest that the polySia chains can influence the transmembrane potential of neural cell membranes in a similar way. This analysis also describes the effect of the transmembrane translocation of negatively charged polyanionic polynucleotydes on the cell membrane potential.