Skip to main content
Log in

Interaction of polypyridyl Cr(III) complexes with bovine serum albumin

  • Original Article
  • Published:
European Biophysics Journal Aims and scope Submit manuscript

Abstract

We report a detailed investigation of the interaction of Cr(NN)33+ with bovine serum albumin (BSA), an important protein for the transport of drugs in blood plasma which allows us to understand further the role of Cr(NN)33+ as a sensitizer in photodynamic therapy (PDT). Chromium(III) complexes, Cr(5Cl-phen)33+, Cr(5Me-phen)33+ and Cr(5Ph-phen)33+ (where Cl = chlorine, Me = methyl and Ph = phenyl are substituents in position 5 of the phen = 1,10-phenanthroline bidentate ligand), were used for the present study. The interactions of BSA with Cr(NN)33+ were assessed employing fluorescence spectroscopy and UV–Vis absorption spectroscopy; in addition electrochemical experiments carried out at a liquid/liquid interface gave insight into the relative hydrophobicities of the complexes. We found that chromium complexes bind strongly with bovine serum albumins (BSA) with intrinsic binding constants, Kb, of (3.33 ± 0.08) × 105 M−1, (5.92 ± 0.08) × 105 M−1 and (1.64 ± 0.05) × 105 M−1 at 300.3 K. Analysis of the thermodynamic parameters ΔG, ΔH, and ΔS indicated that hydrophobic interactions played a major role in all the BSA-Cr(NN)33+ association processes. The binding distances and transfer efficiencies for BSA binding reactions were calculated according to the Förster theory of non-radiation energy transfer giving distance (r) of 2.63 nm, 2.94 nm and 3.00 nm for 5Clphen, 5Mephen and 5Ph phenanthroline complexes, respectively. All these experimental results indicate that Cr(NN)33+ binds to serum albumins, by which these proteins could act as carriers of this complex for further applications in PDT.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or Ebook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7

Similar content being viewed by others

Abbreviations

Cr(5Cl-phen)33+ :

Tris(5-chloro-1,10-phenanthroline) chromium (III) complex

Cr(5Ph-phen)33+ :

Tris(5-phenyl-1,10-phenanthroline) chromium (III) complex

Cr(5Me-phen)33+ :

Tris(5-methyl-1,10-phenanthroline) chromium (III) complex

BSA:

Bovine serum albumin

PDT:

Photodynamic therapy

FRET:

Fluorescence resonant energy transfer

ITIES:

Interface between two immiscible electrolyte solutions

References

Download references

Acknowledgements

The authors thank Consejo Nacional de Investigaciones Científicas y Técnicas de Argentina (CONICET) (Grant number PIP 2015-2017 11220150100628CO), Secretaría de Ciencia y Técnica de la Universidad Nacional de Córdoba (SeCyT-UNC) and Agencia Nacional de Promoción de la Ciencia y la Técnica (ANPCyT) for financial support. P.F.G and C.C.A. are research fellows from CONICET. G.A.A., J.R. and L.M.Y. are researchers from CONICET.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Gerardo A. Argüello.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Garcia, P.F., Coronel Arrechea, C., Velo, A. et al. Interaction of polypyridyl Cr(III) complexes with bovine serum albumin. Eur Biophys J 49, 125–132 (2020). https://doi.org/10.1007/s00249-019-01416-w

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00249-019-01416-w

Keywords

Navigation