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Inhibition of influenza virus activity by the bovine seminal plasma protein PDC-109

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Abstract

A number of viruses causing sexually transmissible diseases are transmitted via mammalian seminal plasma. Several components of seminal plasma have been shown to influence those viruses and their physiological impact. To unravel whether components of seminal plasma could affect viruses transmitted via other pathways, it was investigated here whether the bovine seminal plasma protein PDC-109, belonging to the Fn-type 2 protein family, influences the activity of influenza A viruses, used as a model for enveloped viruses. We found that PDC-109 inhibits the fusion of influenza virus with human erythrocyte membranes and leads to a decreased viral infection in MDCK cells. In the presence of the head group of the phospholipid phosphatidylcholine, phosphorylcholine, the inhibitory effect of PDC-109 was attenuated. This indicates that the impact of the protein is mainly caused by its binding to viral and to erythrocyte membranes thereby interfering with virus-cell binding. Our study underlines that Fn-type 2 proteins have to be considered as new antiviral components present in mammalian seminal plasma.

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Acknowledgements

The work was supported by the German Ministry of Research and Education (BMBF) (e:BioViroSign).

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Author notes

  1. Robert-William Welke

    Present address: Department of Intracellular Proteolysis, Max Delbrück Center for Molecular Medicine, Robert-Rössle-Str. 10, 13125, Berlin, Germany

  2. Ivan Haralampiev

    Present address: Department of Crystallography, Max Delbrück Center for Molecular Medicine, Robert-Rössle-Str. 10, 13125, Berlin, Germany

  3. Filip Schröter

    Present address: Department of Cardiovascular Surgery, Heart Center Brandenburg, Brandenburg Medical School “Theodor-Fontane”, Ladeburger Straße 17, 16321, Bernau, Germany

  4. Christian Sieben

    Present address: Laboratory for Experimental Biophysics, School of Basic Sciences, École Polytechnique Fédérale de Lausanne (EPFL), 1015, Lausanne, Switzerland

Authors and Affiliations

  1. Institute for Biology, Humboldt-Universität zu Berlin, Invalidenstr. 42, 10115, Berlin, Germany

    Robert-William Welke, Ivan Haralampiev, Andreas Herrmann, Christian Sieben & Peter Müller

  2. Leibniz Institute for Zoo and Wildlife Research, Alfred-Kowalke-Straße 17, 10315, Berlin, Germany

    Filip Schröter & Beate C. Braun

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  1. Robert-William Welke
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Welke, RW., Haralampiev, I., Schröter, F. et al. Inhibition of influenza virus activity by the bovine seminal plasma protein PDC-109. Eur Biophys J 48, 503–511 (2019). https://doi.org/10.1007/s00249-019-01374-3

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