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European Biophysics Journal

, Volume 48, Issue 4, pp 349–359 | Cite as

Low cost non-electromechanical technique for the purification of giant unilamellar vesicles

  • Mohammad Abu Sayem KaralEmail author
  • Mostafizur Rahman
  • Md. Kabir Ahamed
  • Sayed Ul Alam Shibly
  • Marzuk Ahmed
  • Md. Mostofa Shakil
Original Article

Abstract

Lipid membranes of giant unilamellar vesicles (GUVs) with diameters greater than 10 μm are promising model membranes for investigating the physical and biological properties of the biomembranes of cells. These are extensively used for the study of the interaction of various membrane-active agents, where purified and similar-size oil-free GUVs are necessary. Although the existing membrane filtering method provides the required quality and quantity of GUVs, it includes a relatively expensive double-headed peristaltic pump. In our proposed non-electromechanical technique, gravity is used to maintain the flow of buffer, wherein the flow rate of buffer with the suspension of GUVs is controlled by a locally available low cost roller clamp regulator. We have characterized the results of this non-electromechanical approach in terms of size distribution, average size, flow rate and efficiency for dioleoylphosphatidylglycerol (DOPG)/dioleoylphosphatidylcholine (DOPC)-GUVs prepared by the natural swelling method. The technique purifies the GUVs by removing the non-entrapped solutes at an optimum flow rate 1.0–2.0 mL/min. In addition, it gives similar results to the pump-driven membrane filtering method. Therefore, it might be a cost effective technique for the purification of GUVs without employing any electromechanical devices.

Keywords

Purification Non-electromechanical GUVs Low cost Membranes 

Notes

Acknowledgements

This work was supported partly by the Grants from Ministry of Science and Technology (39.00.0000.09.02.69.16-17/Phy’s-370 and 39.00.0000.09.06.79.2017/Phy’s-450), Ministry of Education (7.20.0000.004.033.020.2016.1478), Directorate of Advisory, Extension and Research Services, BUET (R-01/2017/2310-73) and University Grants Commission (UGC/ST/Phys-9/2017) of Bangladesh.

Compliance with ethical standards

Conflict of interest

The authors declare no conflict of interest.

Supplementary material

249_2019_1363_MOESM1_ESM.doc (370 kb)
Supplementary material 1 (DOC 370 kb)

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Copyright information

© European Biophysical Societies' Association 2019

Authors and Affiliations

  • Mohammad Abu Sayem Karal
    • 1
    Email author
  • Mostafizur Rahman
    • 1
  • Md. Kabir Ahamed
    • 1
  • Sayed Ul Alam Shibly
    • 2
  • Marzuk Ahmed
    • 1
  • Md. Mostofa Shakil
    • 1
  1. 1.Biophysics Research Laboratory, Department of PhysicsBangladesh University of Engineering and TechnologyDhakaBangladesh
  2. 2.Department of Basic SciencePrimeasia UniversityDhakaBangladesh

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