European Biophysics Journal

, Volume 47, Issue 8, pp 961–966 | Cite as

Affinity determination of biomolecules: a kinetic model for the analysis of pre-equilibrium titration curves

  • Emilie Vanhove
  • Marc Vanhove
Methods Paper


Equilibrium titration curves have been used to determine the affinity of biomolecules using a variety of platforms and technologies. Such measurements, however, are experimentally limited by the time that is needed for the system to reach the equilibrium, which may exceed the time during which the studied biomolecules are stable. We propose here a kinetic model to analyze titration curves obtained prior to full equilibration of the system.


Affinity measurements Pre-equilibrium titration curves High-affinity interactions Therapeutic antibodies 


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Copyright information

© European Biophysical Societies' Association 2018

Authors and Affiliations

  1. 1.Faculté des Sciences AppliquéesInstitut de Mathématique (B37), Quartier Polytech 1LiègeBelgium
  2. 2.Marc Vanhove ConsultancyBoncellesBelgium
  3. 3.ThromboGenics N.V.LeuvenBelgium

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