European Biophysics Journal

, Volume 47, Issue 5, pp 591–596 | Cite as

NMR comparison of hyphal and yeast Candida albicans serotype B mannans

  • Peter Bystrický
  • Eva Machová
  • Slavomír Bystrický
Biophysics Letter


A change from a globular to a filamentous hyphal form is an important feature in the pathogenicity of yeasts. Such a dimorphism while infecting a host organism is thought to be also accompanied in the case of Candida albicans spp. by a structural rearrangement of surface mannan antigen. The presented work brings new insights into the molecular structural changes of mannan C. albicans serotype B based on NMR experimental data. 1H and 13C signal identification of the anomeric region and the assignment of their linkage type is presented here. 2D deconvolution of the HSQC spectra facilitated accurate integration of all anomeric cross-peaks. Analysis of the differences in the integrals led to the proposal that C. albicans serotype B hyphal mannan side chains have the shortened structural moieties: Manα1-2Manα1- and Manα1-3 [Manα1-6] Manα1-2Manα1-. These represent the dominant structures important for construction of a saccharide-based prospective anti-candida vaccine.


NMR Yeast Hyphae Candida albicans Serotype A Serotype B Mannan 



This work was supported by the project ‘‘Biomedical Center Martin’’, ITMS code: 26220220187 co-financed from EU sources. The work was also supported by the Slovak Research and Development Agency under the Contract No. APVV-15-0161 and VEGA Grants Nos. 2/0098/17 and 1/0206/18.


  1. Bystrický P, Dobrota D, Račay P, Bystrický S (2017) NMR characteristics of α-d-Man-(1 → 2)-d-Man and α-d-Man-(1 → 3)-d-Man mannobioses related to Candida albicans yeast mannan structures. Chem Pap 71:2485–2493CrossRefGoogle Scholar
  2. Castanar L, Sistare E, Virgili A, Williamson RT, Parella T (2015) Suppression of phase and amplitude J(HH) modulations in HSQC experiments. Mag Res Chem 53:115–119CrossRefGoogle Scholar
  3. Chorvatovičová D, Machová E, Šandula J, Kogan G (1999) Protective effect of the yeast glucomannan against cyclophosphamide-induced mutagenicity. Mut Res 444:117–122CrossRefGoogle Scholar
  4. Han Y, Morrison RP, Cutler JE (1998) A vaccine and monoclonal antibodies that enhance mouse resistance to Candida albicans vaginal infection. Infect Immun 66:5771–5776PubMedPubMedCentralGoogle Scholar
  5. Jones JKN, Stoodley RJ (1965) Fractionation using copper complexes. Meth Carbohydr Chem 5:36–38Google Scholar
  6. Ližičárová I, Matulová M, Capek P, Machová E (2007) Human pathogen Candida dubliniensis: A cell wall mannan with a high content of β-1,2-linked mannose residues. Carbohydr Polym 70:89–100CrossRefGoogle Scholar
  7. Machová E, Bystrický S (2012) Yeast mannans protect liposomes against peroxidation but do not scavenge free radicals. Carbohydr Polym 88:793–797CrossRefGoogle Scholar
  8. Machová E, Fiačanová L, Čížová A, Korcová J (2015) Mannoproteins from yeast and hyphal form of Candida albicans considerably differ in mannan and protein content. Carbohydr Res 408:12–17CrossRefPubMedGoogle Scholar
  9. Mochon AB, Cutler JE (2005) Prospects of vaccines for medically important fungi. Med Mycol 43:97–115CrossRefPubMedGoogle Scholar
  10. Okawa Y, Takahata T, Kawamata M, Miyauchi M, Shibata N, Suzuki A, Kobayashi H, Suzuki S (1994) Temperature dependent change of serological specificity of Candida albicans NIH A-207 cells cultured in yeast extract-added Sabouraud liquid medium: disappearance of surface antigenic factors 4, 5, and 6 at high temperature. FEBS Lett 345:167–171CrossRefPubMedGoogle Scholar
  11. Paulovičová L, Paulovičová E, Karelin AA, Tsvetkov YE, Nifantiev NE, Bystrický S (2013) Effect of branched α-oligomannoside structures on induction of anti-candida humoral immune response. Scand J Immunol 77:431–441CrossRefPubMedGoogle Scholar
  12. Paulovičová L, Paulovičová E, Bystrický S (2014) Immunological basis of anti-Candida vaccines focused on synthetically prepared cell wall mannan derived mannooligomers. Microbiol Immunol 58:545–551CrossRefPubMedGoogle Scholar
  13. Shibata N, Ikuta K, Imai T, Satoh Y, Satoh R, Suzuki A, Kojima Ch, Kobayashi H, Hisamichi K, Suzuki S (1995) Existence of branched side chains in the cell wall mannan of pathogenic yeast, Candida albicans. J Biolog Chem 270:1113–1122CrossRefGoogle Scholar
  14. Shibata N, Suzuki A, Kobayashi H, Okawa Y (2007) Chemical structure of the cell-wall mannan of Candida albicans serotype A and its difference in yeast and hyphal forms. Biochem J 404:365–372CrossRefPubMedPubMedCentralGoogle Scholar

Copyright information

© European Biophysical Societies' Association 2018

Authors and Affiliations

  1. 1.Division of Neurosciences, Biomedical Center Martin, Jessenius Faculty of Medicine in MartinComenius University in BratislavaMartinSlovakia
  2. 2.Institute of ChemistrySlovak Academy of SciencesBratislavaSlovakia

Personalised recommendations