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Modulating intracellular acidification by regulating the incubation time of proton caged compounds

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Abstract

A proton caged compound, the 1-(2-nitrophenyl)- ethylhexadecyl sulfonate (HDNS), was dosed into HEK-293 at different incubation times. Samples were irradiated with filtered UV light for inducing photolysis of the HDNS and then probed by infrared spectroscopy. The intracellular acidification reaction can be followed by monitoring the consequent CO2 peak intensity variation. The total CO2 produced is similar for all the samples, hence it is only a function of the initial HDNS concentration. The way it is achieved, though, is different for the different incubation times and follows kinetics, which results in a combination of a linear CO2 increase and a steep CO2 increase followed by a decay. This is interpreted in terms of confinement of the HDNS into intracellular vesicles of variable average size and sensitive to UV light when they reach critical dimensions.

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Authors and Affiliations

  1. Department of Chemical Sciences and Technologies, University of Rome Tor Vergata, Via della Ricerca Scientifica, 1, 00133, Rome, Italy

    Marilena Carbone, Gianfranco Sabbatella & Simonetta Antonaroli

  2. Consorzio Interuniversitario Biostrutture e Biosistemi, Viale Medaglie d’Oro 305, 00136, Rome, Italy

    Marilena Carbone

  3. Department of Chemistry, University of Rome La Sapienza, P.le A. Moro, 00185, Rome, Italy

    Gianfranco Sabbatella

  4. Department of Biology and Biotechnology “Charles Darwin”, University of Rome La Sapienza, P.le A. Moro, 00185, Rome, Italy

    Viviana Orlando & Stefano Biagioni

  5. Department of Physics, University of Rome La Sapienza, P.le A. Moro, 00185, Rome, Italy

    Alessandro Nucara

  6. Center for Life Nano Science, Istituto Italiano di Tecnologia, Viale Regina Elena 291, 00161, Rome, Italy

    Alessandro Nucara

Authors
  1. Marilena Carbone
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  2. Gianfranco Sabbatella
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  3. Simonetta Antonaroli
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  4. Viviana Orlando
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  5. Stefano Biagioni
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  6. Alessandro Nucara
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Correspondence to Marilena Carbone.

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Carbone, M., Sabbatella, G., Antonaroli, S. et al. Modulating intracellular acidification by regulating the incubation time of proton caged compounds. Eur Biophys J 45, 565–571 (2016). https://doi.org/10.1007/s00249-016-1122-5

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  • DOI: https://doi.org/10.1007/s00249-016-1122-5

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