Macrophage adhesion on fibronectin evokes an increase in the elastic property of the cell membrane and cytoskeleton: an atomic force microscopy study
- 629 Downloads
Interactions between cells and microenvironments are essential to cellular functions such as survival, exocytosis and differentiation. Cell adhesion to the extracellular matrix (ECM) evokes a variety of biophysical changes in cellular organization, including modification of the cytoskeleton and plasma membrane. In fact, the cytoskeleton and plasma membrane are structures that mediate adherent contacts with the ECM; therefore, they are closely correlated. Considering that the mechanical properties of the cell could be affected by cell adhesion-induced changes in the cytoskeleton, the purpose of this study was to investigate the influence of the ECM on the elastic properties of fixed macrophage cells using atomic force microscopy. The results showed that there was an increase (~50 %) in the Young’s modulus of macrophages adhered to an ECM-coated substrate as compared with an uncoated glass substrate. In addition, cytochalasin D-treated cells had a 1.8-fold reduction of the Young’s modulus of the cells, indicating the contribution of the actin cytoskeleton to the elastic properties of the cell. Our findings show that cell adhesion influences the mechanical properties of the plasma membrane, providing new information toward understanding the influence of the ECM on elastic alterations of macrophage cell membranes.
KeywordsAtomic force microscopy Elastic modulus Nanoindentation Extracellular matrix Cytoskeleton Fibronectin
This research was supported by CAPES/Nanobiotecnologia, Pró-equipamentos/PROCAD, Pronex/FAPEAL, CNPq and FINEP.
- Bukharaev AA, Mozhanova AA, Nurgazizov NI, Ovchinnikov DV (2003) Measuring local elastic properties of cell surfaces and soft materials in liquid by atomic force microscopy. Phys Low-Dimens Str 3–4:31–37Google Scholar
- Hertz H (1881) Ueber den kontakt elastischer koerper. J Reine Angew Math 92:156–171Google Scholar
- Horcas I, Fernandez R, Gomez-Rodriguez JM, Colchero J, Gomez- Herrero J, Baro AM (2007) WSXM: A software for scanning probe microscopy and a tool for nanotechnology. Rev Sci Instrum 78Google Scholar
- Ingber DE, Dike L, Hansen L, Karp S, Liley H, Maniotis A, Mcnamee H, Mooney D, Plopper G, Sims J, Wang N (1994) Cellular tensegrity—exploring how mechanical changes in the cytoskeleton regulate cell-growth, migration, and tissue pattern during morphogenesis. Int Rev Cytol 150:173–224PubMedCrossRefGoogle Scholar
- Morris CE, Homann U (2001) Cell surface area regulation and membrane tension. J Membrane Biol 179:79–102Google Scholar
- Mozhanova AA, Nurgazizov NI, Bukharaev AA(2003) Local elastic properties of biological materials studied by SFM. SPM-2003 In: Proceedings, Nizhni Novgorod, pp 266–267Google Scholar